College of Food Sciences and Technology, South China University of Technology, Guangzhou 510640, China.
Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
Microb Pathog. 2017 Oct;111:285-291. doi: 10.1016/j.micpath.2017.08.005. Epub 2017 Aug 9.
Biofilm is a ubiquitous growth pattern of bacterial species survival but is notorious for its threat on public health and food contamination. Extensive studies of the biofilm structure, formation, quantification, quorum sensing system and underlying control strategies have been reported during the past decades. Insightful elucidation of the pathogenic features and characteristic of bacterial biofilm can facilitate in devising appropriate control strategies for biofilm eradication. Therefore, this review mainly summarized the pathogenic features of biofilms from food borne microorganisms, including the biomass (which could be quantified using crystal violet and fluorogenic dye Syto9 assays), viability (which could be determined by tetrazolium salts, fluorescein diacetate, resazurin staining and alamar blue assays) and matrix (which are commonly detected by dimethyl methylene blue and wheat germ agglutinin assays). In addition, three features were further compared with its particular benefits in specific application.
生物膜是细菌物种生存的一种普遍存在的生长模式，但它对公共卫生和食物污染的威胁却是臭名昭著的。在过去的几十年里，人们对生物膜的结构、形成、定量、群体感应系统和潜在的控制策略进行了广泛的研究。深入了解细菌生物膜的致病特征有助于制定适当的生物膜清除控制策略。因此，本综述主要总结了食源微生物生物膜的致病特征，包括生物量（可通过结晶紫和荧光染料 Syto9 测定）、活性（可通过四唑盐、荧光素二乙酸酯、resazurin 染色和 alamar blue 测定）和基质（通常通过二甲亚甲基蓝和麦胚凝集素测定）。此外，还进一步比较了这三个特征在特定应用中的特殊优势。
