Gao Danfeng, Zhang Xiufen, Liu Beibei, Meng Dong, Fang Kai, Guo Zijian, Li Lihua
Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi 214062, China.
Department of Oncological Surgery, The Affiliated Hospital of Jiangnan University, Wuxi 214062, China.
Epigenomics. 2017 Sep;9(9):1175-1188. doi: 10.2217/epi-2017-0055. Epub 2017 Aug 14.
We aimed to identify circular RNAs (circRNAs) associated with breast cancer chemoresistance.
MATERIALS & METHODS: CircRNA microarray expression profiles were obtained from Adriamycin (ADM) resistant MCF-7 breast cancer cells (MCF-7/ADM) and parental MCF-7 cells and were validated using quantitative real-time reverse transcription PCR. The expression data were analyzed bioinformatically.
We detected 3093 circRNAs and identified 18 circRNAs that are differentially expressed between MCF-7/ADM and MCF-7 cells; after validating by quantitative real-time reverse transcription PCR, we predicted the possible miRNAs and potential target genes of the seven upregulated circRNAs using TargetScan and miRanda. The bioinformatics analysis revealed several target genes related to cancer-related signaling pathways. Additionally, we discovered a regulatory role of the circ_0006528-miR-7-5p-Raf1 axis in ADM-resistant breast cancer.
These results revealed that circRNAs may play a role in breast cancer chemoresistance and that hsa_circ_0006528 might be a promising candidate for further functional analysis.
我们旨在鉴定与乳腺癌化疗耐药相关的环状RNA（circRNA）。
从阿霉素（ADM）耐药的MCF-7乳腺癌细胞（MCF-7/ADM）和亲本MCF-7细胞中获得circRNA微阵列表达谱，并使用定量实时逆转录PCR进行验证。对表达数据进行生物信息学分析。
我们检测到3093个circRNA，并鉴定出18个在MCF-7/ADM和MCF-7细胞之间差异表达的circRNA；通过定量实时逆转录PCR验证后，我们使用TargetScan和miRanda预测了7个上调的circRNA可能的miRNA和潜在靶基因。生物信息学分析揭示了几个与癌症相关信号通路相关的靶基因。此外，我们发现circ_0006528-miR-7-5p-Raf1轴在ADM耐药乳腺癌中具有调节作用。
这些结果表明，circRNA可能在乳腺癌化疗耐药中发挥作用，并且hsa_circ_0006528可能是进一步功能分析的有希望的候选者。
