Goldkuhl R, Klockars A, Carlsson H-E, Hau J, Abelson K S P
Division of Comparative Medicine, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
Eur Surg Res. 2010;44(2):117-23. doi: 10.1159/000264962. Epub 2010 Feb 9.
Tissue injury and anaesthesia during surgery induce a stress response associated with increased glucocorticoid secretion from the adrenal glands. This response alters the normal physiology and may cause postoperative morbidity, as well as affect the results during acute experiments. The aim of the present investigation was to study the effect of surgical severity and analgesic treatment on circulating corticosterone in male Sprague-Dawley rats. Male rats were treated with either lidocaine infiltrated during surgery, buprenorphine (0.05 or 0.1 mg/kg subcutaneously) or saline subcutaneously. Each treatment group was subjected to either arterial catheterisation or arterial catheterisation and laparotomy. A catheter was inserted in the common carotid artery and blood was collected during surgery and during anaesthesia 6 h after surgery. Lidocaine treatment reduced the corticosterone levels compared to saline treatment after catheterisation but not after laparotomy. Buprenorphine treatment reduced the corticosterone levels during the first hour after surgery after both catheterisation and laparotomy. The higher buprenorphine dose led to an earlier and more pronounced reduction, especially after laparotomy. In the present study, the corticosterone response during surgery in laboratory rats is correlated with the severity of the procedure, and buprenorphine reduces the surgical stress response more effectively than lidocaine treatment.
手术过程中的组织损伤和麻醉会引发一种应激反应，该反应与肾上腺糖皮质激素分泌增加有关。这种反应会改变正常生理状态，可能导致术后发病，还会影响急性实验的结果。本研究的目的是探讨手术严重程度和镇痛治疗对雄性斯普拉格-道利大鼠循环皮质酮的影响。雄性大鼠在手术期间接受利多卡因浸润、丁丙诺啡（皮下注射0.05或0.1毫克/千克）或皮下注射生理盐水治疗。每个治疗组分别接受动脉插管或动脉插管及剖腹手术。将导管插入颈总动脉，并在手术期间及术后6小时麻醉期间采集血液。与插管后生理盐水治疗相比，利多卡因治疗降低了皮质酮水平，但剖腹手术后未降低。丁丙诺啡治疗在插管和剖腹手术后的术后第一小时内降低了皮质酮水平。丁丙诺啡较高剂量导致更早、更明显的降低，尤其是在剖腹手术后。在本研究中，实验大鼠手术期间的皮质酮反应与手术程序的严重程度相关，丁丙诺啡比利多卡因治疗更有效地降低手术应激反应。
