通过功能测试对RNA聚合酶III启动子进行界定。
Delimitation of a promoter for RNA polymerase III by means of a functional test.
作者信息
Telford J L, Kressmann A, Koski R A, Grosschedl R, Müller F, Clarkson S G, Birnstiel M L
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2590-4. doi: 10.1073/pnas.76.6.2590.
Abstract
A Xenopus laevis DNA segment containing the structural gene for tRNA1Met and 22 base pairs at the 5' side of the gene is active in tRNA production. The DNA segment was ligated to sea urchin histone DNA and was also inserted into plasmid pCR1. Both recombinant DNAs were shown to produce mature tRNA1Met at a high rate when injected into centrifuged Xenopus oocytes.
摘要
一段包含tRNA1Met结构基因及其基因5'端22个碱基对的非洲爪蟾DNA片段在tRNA产生过程中具有活性。该DNA片段与海胆组蛋白DNA连接，并插入质粒pCR1中。当将这两种重组DNA注射到经离心处理的非洲爪蟾卵母细胞中时，它们都能高效产生成熟的tRNA1Met。
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