Brahmi Soumia, Touati Abdelaziz, Dunyach-Remy Catherine, Sotto Albert, Pantel Alix, Lavigne Jean-Philippe
1 Laboratoire d'Écologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia , Bejaia, Algérie.
2 UFR de Médecine, Institut National de la Santé et de la Recherche Médicale, U1047, Université de Montpellier , Nîmes, France .
Microb Drug Resist. 2018 Apr;24(3):290-298. doi: 10.1089/mdr.2017.0149. Epub 2017 Aug 14.
We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among wild fish from the coast of Bejaia (Algeria) in the Mediterranean Sea.
From March 2012 to August 2013, gut and gill samples of wild fish were screened for the presence of ESBL-producing Enterobacteriaceae. Strains were characterized with regard to antibiotic resistance, β-lactamase content, plasmid-mediated quinolone resistance, aminoglycoside resistance genes, and clonality (repetitive sequence-based polymerase chain reaction profiles and multilocus sequence typing). Virulence traits were performed for Escherichia coli and Klebsiella pneumoniae isolates. Of the 300 fish studied, 64 (21.3%) isolates were screened as positive for ESBL producing by the double-disc method. The isolates corresponded to E. coli, K. pneumoniae, Enterobacter cloacae, Morganella morganii, Citrobacter freundii, and Proteus vulgaris. A predominance of bla gene was observed with a prevalence of 60.5% (n = 46). Furthermore, our study describes the association of important coresistance and virulence factors in E. coli and K. pneumoniae. Twelve of the ESBL producers carried genes of the qnr family and oqxAB gene and six carried the aac(6')-Ib-cr gene.
Our results highlight for the first time the diffusion of multidrug-resistant Enterobacteriaceae isolates carrying resistance and virulence genes in fish from the Mediterranean Sea in Algeria.
我们调查了地中海阿尔及利亚贝贾亚海岸野生鱼类中产超广谱β-内酰胺酶（ESBL）肠杆菌科细菌的流行情况。
2012年3月至2013年8月，对野生鱼类的肠道和鳃样本进行筛查，以检测产ESBL肠杆菌科细菌的存在。对菌株进行了抗生素耐药性、β-内酰胺酶含量、质粒介导的喹诺酮耐药性、氨基糖苷类耐药基因和克隆性（基于重复序列的聚合酶链反应图谱和多位点序列分型）的鉴定。对大肠杆菌和肺炎克雷伯菌分离株进行了毒力特性检测。在研究的300条鱼中，有64株（21.3%）通过双纸片法筛查为产ESBL阳性。分离株包括大肠杆菌、肺炎克雷伯菌、阴沟肠杆菌、摩根摩根菌、弗氏柠檬酸杆菌和普通变形杆菌。观察到bla基因占优势，流行率为60.5%（n = 46）。此外，我们的研究描述了大肠杆菌和肺炎克雷伯菌中重要的共同耐药和毒力因子的关联。12株产ESBL菌携带qnr家族基因和oqxAB基因，6株携带aac(6')-Ib-cr基因。
我们的结果首次突出了阿尔及利亚地中海海域鱼类中携带耐药和毒力基因的多重耐药肠杆菌科分离株的扩散情况。
