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川芎根茎中一种补体固定多糖的纯化及部分结构表征

Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong.

作者信息

Zou Yuan-Feng, Fu Yu-Ping, Chen Xing-Fu, Austarheim Ingvild, Inngjerdingen Kari Tvete, Huang Chao, Eticha Lemlem Dugassa, Song Xu, Li Lixia, Feng Bin, He Chang-Liang, Yin Zhong-Qiong, Paulsen Berit Smestad

机构信息

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China.

Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China.

出版信息

Molecules. 2017 Feb 14;22(2):287. doi: 10.3390/molecules22020287.

DOI:10.3390/molecules22020287
PMID:28216596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6155779/
Abstract

Rhizome of is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator.

摘要

某植物的根茎是一种有效的药用植物，在中国，它已被广泛应用于偏头痛和心血管疾病的治疗达数百年之久。该植物的多糖已显示出有趣的生物活性，但先前的研究仅针对中性多糖进行。在本研究中，从某植物根茎的100℃水提取物中获得了一种果胶多糖组分LCP-I-I，并通过二乙氨基乙基（DEAE）琼脂糖阴离子交换色谱和凝胶过滤进行纯化。除傅里叶变换红外（FT-IR）光谱仪和核磁共振（NMR）光谱外，单糖分析和连接测定表明LCP-I-I是一种典型的果胶多糖，具有同型半乳糖醛酸聚糖和鼠李糖半乳糖醛酸聚糖I型区域以及阿拉伯半乳聚糖I型和II型（AG-I/AG-II）侧链。LCP-I-I表现出强大的补体固定活性，半数抑制浓度（ICH）为26.3±2.2μg/mL，因此具有作为天然免疫调节剂的潜力。 （注：原文中部分植物名称未给出具体信息，翻译时用“某植物”代替）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/78711bb1fb0d/molecules-22-00287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/bbeb588ce146/molecules-22-00287-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/13bbcce161ca/molecules-22-00287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/0d29a95d58ea/molecules-22-00287-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/3d6fead7ae0f/molecules-22-00287-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/78711bb1fb0d/molecules-22-00287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/bbeb588ce146/molecules-22-00287-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/13bbcce161ca/molecules-22-00287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/0d29a95d58ea/molecules-22-00287-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/3d6fead7ae0f/molecules-22-00287-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca5b/6155779/78711bb1fb0d/molecules-22-00287-g005.jpg

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