党参根多糖的结构特征及补体固定活性。
Structural features and complement fixing activity of polysaccharides from Codonopsis pilosula Nannf. var. modesta L.T.Shen roots.
机构信息
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, PR China.
出版信息
Carbohydr Polym. 2014 Nov 26;113:420-9. doi: 10.1016/j.carbpol.2014.07.036. Epub 2014 Jul 24.
Two pectic polysaccharides, 50 WCP-II-I and 100 WCP-II-I, were obtained from 50 and 100 °C water extracts of Codonopsis pilosula roots by ion exchange chromatography and gel filtration. The study of the sub-fractions obtained after pectinase degradation showed that the complement fixation activities of these pectins are expressed mainly by their ramified regions. The structure studies of native and sub-fractions showed the 50 WCP-II-I is a pectic polysaccharide, with long homogalacturonan regions (some of the galacturonic acid units were methyl esterified), interrupted by one short rhamnogalacturonan I (RG-I) region. The side chains of the RG-I region are arabinogalactan type I (AG-I) and type II (AG-II) attached on position 4 of rhamnose. The 100 WCP-II-I has two main ramified regions, one is galacturonan region with AG-I side chain on position 2 of GalA, and the other one is RG-I region with AG-II side chain on position 4 of Rha.
从党参根的 50°C 和 100°C 水提物中，通过离子交换色谱和凝胶过滤分别得到两种果胶多糖 50 WCP-II-I 和 100 WCP-II-I。对果胶酶降解得到的级分的研究表明，这些果胶的补体固定活性主要由其支化区域表达。天然和级分的结构研究表明，50 WCP-II-I 是一种果胶多糖，具有长的均半乳糖醛酸区域（部分半乳糖醛酸单元被甲酯化），由一个短的鼠李半乳糖醛酸 I（RG-I）区域打断。RG-I 区域的侧链是阿拉伯半乳糖聚糖 I（AG-I）和 II（AG-II），分别连接在鼠李糖的 4 位。100 WCP-II-I 有两个主要的支化区域，一个是半乳糖醛酸区域，AG-I 侧链连接在 GalA 的 2 位，另一个是 RG-I 区域，AG-II 侧链连接在 Rha 的 4 位。
Zotero 插件