Department of Chemistry, Northeastern University , Shenyang 110004, China.
Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University , Fuzhou 350004, China.
ACS Appl Mater Interfaces. 2017 Sep 6;9(35):29881-29888. doi: 10.1021/acsami.7b09861. Epub 2017 Aug 23.
Compared to natural enzymes, nanomaterial-based artificial enzymes have attracted immense attention because of their high stability and cost-effectiveness. In this study, cobalt nitride (CoN) as a noble-metal-free artificial enzyme exhibiting highly intrinsic peroxidase-like activity and good stability was reported. Kinetic studies revealed that the resultant CoN nanowires (NWs) exhibited a stronger affinity for 3,3',5,5'-tetramethylbenzidine (TMB) and HO than HRP. Compared to CoO NWs, CoN NWs exhibited highly improved catalytic activities, with HO exhibiting an apparent K approximately 2 orders of magnitude less than that of CoO. In particular, the peroxidase-like activity of CoN was maintained well over a wide range of temperatures and ionic strength. A CoN-based method was further developed for the detection of glucose with good sensitivity and reliability. Because of advantages such as easy storage, cost-effectiveness, high sensitivity, and outstanding stability, CoN NWs demonstrate the potential for replacing noble-metal-based peroxidase mimetics in a wide range of promising applications.
相较于天然酶，纳米材料基人工酶由于其高稳定性和成本效益而受到了极大关注。在这项研究中，报道了一种具有固有过氧化物酶样活性和良好稳定性的无贵金属钴氮化物（CoN）作为人工酶。动力学研究表明，所得的 CoN 纳米线（NWs）对 3,3',5,5'-四甲基联苯胺（TMB）和 HO 的亲和力更强，超过了 HRP。与 CoO NWs 相比，CoN NWs 表现出了显著提高的催化活性，HO 的表观 K 值大约比 CoO 小 2 个数量级。特别是，CoN 的过氧化物酶样活性在很宽的温度和离子强度范围内都保持良好。进一步开发了基于 CoN 的葡萄糖检测方法，具有良好的灵敏度和可靠性。由于易于储存、成本效益高、灵敏度高和稳定性出色等优点，CoN NWs 有望在广泛的应用领域中替代基于贵金属的过氧化物模拟酶。
