College of Life Science, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Mudanjiang Branch of Heilongjiang Academy of Forestry Science, No. 16, East Diming Street, Mudanjiang, 157010, China.
Appl Microbiol Biotechnol. 2020 May;104(9):3947-3957. doi: 10.1007/s00253-020-10520-3. Epub 2020 Mar 16.
A sensing methodology that combines Au, tobacco mosaic virus (TMV), and folic acid for selective, sensitive, and colorimetric detection of tumor cells based on the peroxidase-like activity was reported in this study. Gold nanowires with a high aspect ratio were synthesized using TMV as a template. Au@TMV nanowire (AT) complex was obtained with diameter of 4 nm and length between 200 and 300 nm. In addition, since TMV was biocompatible and had many amino and carboxyl groups on its surface, AT was conjugated by folate to form a folic acid (FA)-conjugated AT composite (ATF) and tested by FTIR measurements. Furthermore, the peroxidase-like properties were studied and the optimal conditions for mimic enzyme activity were optimized. Finally, HeLa and other tumor cells expressed excessive receptors of folate on the surface, which can specifically bind to folic acid. As the specific binding of ATF with HeLa cells, the peroxidase properties of ATF were used for detection of cancer cells (Scheme 1). The cancer cells were detected not only qualitatively but also quantitatively. In this study, as low as 2000 cancer cells/mL could be detected using the current method.
本研究报道了一种基于过氧化物酶样活性的、结合金、烟草花叶病毒（TMV）和叶酸的传感方法，用于选择性、灵敏性和比色检测肿瘤细胞。使用 TMV 作为模板合成了高纵横比的金纳米线。获得了直径为 4nm 且长度在 200-300nm 之间的 Au@TMV 纳米线（AT）复合物。此外，由于 TMV 具有生物相容性且表面有许多氨基和羧基，因此通过叶酸将 AT 进行偶联形成叶酸（FA）偶联的 AT 复合物（ATF），并通过傅里叶变换红外光谱（FTIR）测量进行测试。进一步研究了过氧化物酶样性质，并优化了模拟酶活性的最佳条件。最后，HeLa 和其他肿瘤细胞表面表达了过量的叶酸受体，能够与叶酸特异性结合。由于 ATF 与 HeLa 细胞的特异性结合，因此可以利用 ATF 的过氧化物酶性质来检测癌细胞（方案 1）。不仅可以定性检测癌细胞，还可以定量检测。在本研究中，使用当前方法可以检测低至 2000 个癌细胞/mL。
