Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box332, Shenyang 110819, China.
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA.
Analyst. 2019 Sep 21;144(18):5455-5461. doi: 10.1039/c9an01262a. Epub 2019 Aug 21.
Over the past few years, artificial enzymes have attracted enormous attention due to their high stabilities and cost-effective productions. In this work, metal-organic framework-derived SiW@CoO was synthesized in large quantities by stirring the mixture at ambient temperature and calcination. The obtained SiW@CoO exhibited a highly inherent peroxidase-like activity and excellent stability. Kinetic studies demonstrated that the synthesized SiW@CoO had a strong binding affinity to 3,3',5,5'-tetramethylbenzidine (TMB), stronger than HRP had. Specifically, the peroxidase-like activity of SiW@CoO in an aqueous solution was well maintained after incubation at an elevated temperature, at an extreme pH and for a long time. A SiW@CoO-based method was further developed for HO and one-pot glucose detection with good sensitivity and reliability. The facile synthesis approach is expected to facilitate the practical use of metal-organic frameworks and their derivatives as enzyme mimics in the future.
在过去的几年中，由于其高稳定性和具有成本效益的生产，人工酶引起了极大的关注。在这项工作中，通过在环境温度下搅拌混合物并进行煅烧，大量合成了金属有机骨架衍生的 SiW@CoO。所获得的 SiW@CoO 表现出了高度固有的过氧化物酶样活性和优异的稳定性。动力学研究表明，所合成的 SiW@CoO 对 3,3',5,5'-四甲基联苯胺（TMB）具有很强的结合亲和力，比 HRP 更强。具体而言，SiW@CoO 的过氧化物酶样活性在高温孵育、极端 pH 和长时间孵育后仍能在水溶液中得到很好的保持。进一步开发了基于 SiW@CoO 的方法，用于 HO 和一次性葡萄糖检测，具有良好的灵敏度和可靠性。这种简便的合成方法有望促进金属有机骨架及其衍生物在未来作为酶模拟物的实际应用。
