Bennett Michael S, Trivedi Shubhanshi, Iyer Anita S, Hale J Scott, Leung Daniel T
Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA; and.
Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.
J Leukoc Biol. 2017 Nov;102(5):1261-1269. doi: 10.1189/jlb.4A0317-116R. Epub 2017 Aug 14.
Mucosal-associated invariant T (MAIT) cells are an innate-like T cell subset, restricted by the nonclassic MHC class I-related protein MR1 and enriched at mucosal sites. Human studies have shown an association between MAIT cells and pathogen-specific antibody responses. In this study, we investigate the effect of human MAIT cells on B cells ex vivo. We found that supernatants from microbe- or cytokine-stimulated MAIT cells, when added to purified autologous B cells, increase frequencies of plasmablasts and promote IgA, IgG, and IgM production. We found effects to be mostly MR1-dependent and that the increases in plasmablasts are likely a result of increased differentiation from memory B cells. Furthermore, microbe-activated MAIT cell supernatant contains multiple cytokines known to stimulate B cells, including IL-6, -10, and -21. This study thus provides the first direct evidence of a newly identified role of MAIT cells in providing help to B cells.
黏膜相关恒定T（MAIT）细胞是一类固有样T细胞亚群，受非经典MHC I类相关蛋白MR1限制，在黏膜部位富集。人体研究表明MAIT细胞与病原体特异性抗体反应之间存在关联。在本研究中，我们在体外研究了人MAIT细胞对B细胞的影响。我们发现，当将来自微生物或细胞因子刺激的MAIT细胞的上清液添加到纯化的自体B细胞中时，会增加浆母细胞频率并促进IgA、IgG和IgM的产生。我们发现这些效应大多依赖于MR1，并且浆母细胞的增加可能是记忆B细胞分化增加的结果。此外，微生物激活的MAIT细胞上清液含有多种已知可刺激B细胞的细胞因子，包括IL-6、-10和-21。因此，本研究首次直接证明了MAIT细胞在为B细胞提供帮助方面的新作用。
