Chang Lei, Yin Chun-Yu, Wu Hai-Yin, Tian Bin-Bin, Zhu Yan, Luo Chun-Xia, Zhu Dong-Ya
Institution of Stem Cells and Neuroregeneration, and Department of Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
The Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing, Jiangsu 211166, China.
J Biomed Res. 2017 Jul 13;31(4):306-314. doi: 10.7555/JBR.31.20160138.
Stroke is one of the leading causes of disability and death globally. It occurs when a major artery is occluded in the brain and leads to death of cells within the injured tissue. (+)-Borneol, a simple bicyclic monoterpene extracted from traditional Chinese medicine, is widely used in various types of diseases. However, no study has proved the effects of (+)-borneol on functional recovery from permanent ischemic stroke and the mechanism is still unknown. Here, we report that in the rat model of permanent cerebral ischemia, we found that (+)-borneol (1.0 mg/kg) significantly ameliorated infarct size and neurological scoresvia reducing the expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) in a dose dependent manner. Notably, (+)-borneol showed long-term effects on the improvement of sensorimotor functions in the photothrombotic model of stroke, which decreased the number of foot faults in the grid-walking task and forelimb asymmetry scores in the cylinder task, at least in part through reducing loss of dendritic spines in the length, brunch number and density. These findings suggest that (+)-borneol could serve as a therapeutic target for ischemic stroke.
中风是全球致残和致死的主要原因之一。当大脑中的一条主要动脉被阻塞并导致受损组织内的细胞死亡时，就会发生中风。（+）-冰片是一种从中药中提取的简单双环单萜，广泛用于各种疾病。然而，尚无研究证实（+）-冰片对永久性缺血性中风功能恢复的影响，其机制仍不清楚。在此，我们报告在永久性脑缺血大鼠模型中，我们发现（+）-冰片（1.0mg/kg）通过剂量依赖性降低诱导型一氧化氮合酶（iNOS）和肿瘤坏死因子-α（TNF-α）的表达，显著改善梗死面积和神经学评分。值得注意的是，（+）-冰片对光血栓性中风模型的感觉运动功能改善具有长期作用，这至少部分是通过减少长度、分支数和密度方面的树突棘丢失，从而减少网格行走任务中的足部错误数量和圆筒任务中的前肢不对称评分。这些发现表明（+）-冰片可作为缺血性中风的治疗靶点。
