Martins Frederico S, Borges Leonardo L, Ribeiro Claudia S C, Reifschneider Francisco J B, Conceição Edemilson C
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Brazil.
Escola de Ciências Médicas, Farmacêuticas e Biomédicas, Pontifícia Universidade Católica de Goiás, 74605-010 Goiânia, GO, Brazil.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S375-S379. doi: 10.4103/0973-1296.210127. Epub 2017 Jul 11.
The objective of this study was to compare three capsaicin extraction methods: Shoxlet, Ultrasound-assisted Extraction (UAE), and Shaker-assisted Extraction (SAE) from Habanero pepper, CNPH 15.192.
The different parameters evaluated were alcohol degree, time extraction, and solid-solvent ratio using response surface methodology (RSM).
The three parameters found significant ( < 0.05) were for UAE and solvent concentration and extraction time for SAE. The optimum conditions for the capsaicin UAE and SAE were similar 95% alcohol degree, 30 minutes and solid-liquid ratio 2 mg/mL. The Soxhlet increased the extraction in 10-25%; however, long extraction times (45 minutes) degraded 2% capsaicin.
The extraction of capsaicin was influenced by extraction method and by the operating conditions chosen. The optimized conditions provided savings of time, solvent, and herbal material. Prudent choice of the extraction method is essential to ensure optimal yield of extract, thereby making the study relevant and the knowledge gained useful for further exploitation and application of this resource.
, line CNPH 15.192, possess capsaicin in higher levels when compared with others speciesHigher levels of ethanolic strength are more suitable to obtain a higher levels of capsaicinBox-Behnken design indicates to be useful to explore the best conditions of ultrasound assisted extraction of capsaicin. Nomenclature UAE: Ultrasound-assisted Extraction; SAE: Shaker-assisted Extraction.
本研究的目的是比较三种辣椒素提取方法：索氏提取法、超声辅助提取法（UAE）和振荡辅助提取法（SAE），所用材料为哈瓦那辣椒CNPH 15.192。
使用响应面法（RSM）评估不同参数，包括酒精浓度、提取时间和固液比。
发现对UAE有显著影响（<0.05）的三个参数是溶剂浓度，对SAE有显著影响的是提取时间。辣椒素UAE和SAE的最佳条件相似，酒精浓度95%、提取时间30分钟、固液比2mg/mL。索氏提取法的提取量提高了10 - 25%；然而，较长的提取时间（45分钟）使辣椒素降解了2%。
辣椒素的提取受提取方法和所选操作条件的影响。优化后的条件节省了时间、溶剂和草药材料。谨慎选择提取方法对于确保提取物的最佳产量至关重要，从而使本研究具有相关性，所获得的知识对于进一步开发和应用该资源有用。
与其他品种相比，CNPH 15.192号线辣椒中的辣椒素含量更高。较高的乙醇浓度更适合获得较高水平的辣椒素。Box - Behnken设计表明有助于探索超声辅助提取辣椒素的最佳条件。术语表：UAE：超声辅助提取；SAE：振荡辅助提取。
