Hussein Samia, Mosaad Hala, Rashed Hayam E, Ahmed Shimaa, Ragab Ahmed, Ismail Eman I
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Mol Biol Rep. 2017 Aug;44(4):365-377. doi: 10.1007/s11033-017-4118-z. Epub 2017 Aug 14.
This study aimed to assess the expression of S100A4, Twist and E-cadherin (mRNA and protein) in urothelial bladder cancer, investigate the correlation between them and evaluate their association with the clinicopathological features of the disease. The study included 54 patients diagnosed as urothelial bladder cancer of different stages and grades. The expression levels of S100A4, Twist and E-cadherin (mRNA and protein) in tissue samples were determined by quantitative RT-PCR and immunohistochemistry. The expression of S100A4 and Twist was significantly upregulated while E- cadherin was significantly downregulated in urothelial bladder cancer tissues compared to the adjacent surrounding normal bladder tissues at both mRNA and protein levels (p < 0.001). Expression levels of S100A4 and Twist were significantly higher in recurrent tumor than in non-recurrent tumors (p < 0.001) while the expression level of E-cadherin was significantly lower in recurrent tumors than in non-recurrent tumors at both mRNA and protein levels (p < 0.001). There was a significant positive correlation between S100A4 and Twist expressions (r = 0.875, p < 0.001) while significant negative correlations were found between E- cadherin and S100A4 expressions(r=- 0.803, p < 0.001) and between E-cadherin and Twist (r = -0.809, p < 0.001). Up-regulation of S100A4 and Twist and down-regulation of E-cadherin in urothelial bladder cancer tissues compared to adjacent normal tissues were observed. There was a significant negative correlation between S100A4 and E- cadherin and between E- cadherin and Twist expression. However, there was a significant positive correlation between S100A4 and Twist expressions. Furthermore, the alterations in the gene expression were associated with disease stage and grade.
本研究旨在评估S100A4、Twist和E-钙黏蛋白（mRNA和蛋白）在膀胱尿路上皮癌中的表达，研究它们之间的相关性，并评估它们与该疾病临床病理特征的关联。该研究纳入了54例被诊断为不同分期和分级的膀胱尿路上皮癌患者。通过定量逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法测定组织样本中S100A4、Twist和E-钙黏蛋白（mRNA和蛋白）的表达水平。与相邻的正常膀胱组织相比，膀胱尿路上皮癌组织中S100A4和Twist的表达在mRNA和蛋白水平均显著上调，而E-钙黏蛋白显著下调（p＜0.001）。复发肿瘤中S100A4和Twist的表达水平显著高于非复发肿瘤（p＜0.001），而复发肿瘤中E-钙黏蛋白的表达水平在mRNA和蛋白水平均显著低于非复发肿瘤（p＜0.001）。S100A4和Twist表达之间存在显著正相关（r = 0.875，p＜0.001），而E-钙黏蛋白与S100A4表达之间（r = -0.803，p＜0.001）以及E-钙黏蛋白与Twist之间（r = -0.809，p＜0.001）存在显著负相关。与相邻正常组织相比，观察到膀胱尿路上皮癌组织中S100A4和Twist上调，E-钙黏蛋白下调。S100A4与E-钙黏蛋白之间以及E-钙黏蛋白与Twist表达之间存在显著负相关。然而，S100A4和Twist表达之间存在显著正相关。此外，基因表达的改变与疾病分期和分级相关。
