Eslami Ali, Hajizadeh Moghaddam Akbar, Khanjani Jelodar Sedigheh, Ranjbar Mojtaba
Department of Animal Sciences, Faculty of Sciences, University of Mazandaran, Babolsar, Iran.
Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
IBRO Neurosci Rep. 2025 Mar 12;18:491-497. doi: 10.1016/j.ibneur.2025.03.004. eCollection 2025 Jun.
Phytosome-based nanocarriers have emerged as innovative drug delivery systems in recent years, demonstrating significant potential in the treatment of neurodegenerative disorders. This study aimed to evaluate the therapeutic efficacy of quercetin-loaded nanophytosome (QNP) in modulating the oxido-inflammatory response in a rat model of early life stress (ELS) induced by maternal isolation (MI). To establish the ELS model, male rat pups were isolated from their dam for 3 hours daily from postnatal days 1-9. Following the lactation period (postpartum days 1-21), treatments with quercetin (10 and 40 mg/kg) and QNP (10 and 40 mg/kg) were administered continuously for 21 days. Cognitive behaviors, oxidative stress markers, hippocampal dopamine levels, and mRNA expression of TNF-α and IL-6 were assessed after ELS induction. Treatment with QNP (40 mg/kg) significantly improved cognitive function ( < 0.01), increased hippocampal dopamine levels ( < 0.001), and reduced oxidative stress ( < 0.01) as well as the expression of TNF-α ( < 0.001) and IL-6 ( < 0.001). In conclusion, QNP demonstrates potent hippocampal anti-oxidoinflammatory effects, making it a promising therapeutic candidate for mitigating the adverse effects of maternal isolation-induced early life stress.
近年来，基于植物脂质体的纳米载体已成为创新的药物递送系统，在神经退行性疾病的治疗中显示出巨大潜力。本研究旨在评估负载槲皮素的纳米植物脂质体（QNP）在调节母体隔离（MI）诱导的早期生活应激（ELS）大鼠模型中的氧化炎症反应方面的治疗效果。为建立ELS模型，雄性幼鼠在出生后第1 - 9天每天与母鼠隔离3小时。哺乳期（产后第1 - 21天）后，连续21天给予槲皮素（10和40 mg/kg）和QNP（10和40 mg/kg）进行治疗。在ELS诱导后评估认知行为、氧化应激标志物、海马多巴胺水平以及TNF-α和IL-6的mRNA表达。用QNP（40 mg/kg）治疗可显著改善认知功能（<0.01），提高海马多巴胺水平（<0.001），降低氧化应激（<0.01）以及TNF-α（<0.001）和IL-6（<于0.001）的表达。总之，QNP显示出强大的海马抗氧化炎症作用，使其成为减轻母体隔离诱导的早期生活应激不良影响的有前景的治疗候选物。
