Ireton Renee C, Wilkins Courtney, Gale Michael
Department of Immunology, Center for Innate Immunity and Immune Disease, University of Washington School of Medicine, E383, 750 Republican Street, Seattle, WA, 98109, USA.
Methods Mol Biol. 2017;1656:119-129. doi: 10.1007/978-1-4939-7237-1_6.
Pathogen recognition receptors (PRR)s and their cognate pathogen-associated molecular pattern (PAMP) represent the basis of innate immune activation and immune response induction driven by the host-pathogen interaction that occurs during microbial infection in humans and other animals. For RNA virus infection such as hepatitis C virus (HCV) and others, specific motifs within viral RNA mark it as nonself and visible to the host as a PAMP through interaction with RIG-I-like receptors including retinoic inducible gene-I (RIG-I). Here, we present methods for producing and using HCV PAMP RNA as a molecular tool to study RIG-I and its signaling pathway, both in vitro and in vivo, in innate immune regulation.
病原体识别受体（PRR）及其同源病原体相关分子模式（PAMP）代表了先天性免疫激活和免疫反应诱导的基础，这种激活和诱导是由人类和其他动物在微生物感染期间发生的宿主-病原体相互作用驱动的。对于丙型肝炎病毒（HCV）等RNA病毒感染，病毒RNA中的特定基序将其标记为非己成分，并通过与包括视黄酸诱导基因-I（RIG-I）在内的RIG-I样受体相互作用，作为PAMP被宿主识别。在这里，我们介绍了生产和使用HCV PAMP RNA作为分子工具的方法，以在体外和体内研究先天性免疫调节中RIG-I及其信号通路。
