Chen Wen-Ming, Li Yi-Shu, Young Chiu-Chung, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd., Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2689-2695. doi: 10.1099/ijsem.0.001993. Epub 2017 Aug 15.
A bacterial strain, designated gyp-1T, was isolated from a mangrove in Taiwan and characterized using the polyphasic taxonomic approach. Cells of gyp-1T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, coccoid or short-rod-shaped and formed cream-coloured colonies. Growth occurred at 15-37 °C (optimum, 25-30 °C), at pH 5.5-7.0 (optimum, pH 6.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences indicated that gyp-1T represented a member of the genus Paracoccus and showed the highest levels of sequence similarity with respect to Paracoccus lutimaris HDM-25T (97.8 %) and Paracoccus aminovorans DM-82T (97.7 %). The major fatty acids (>10 %) of gyp-1T were C18 : 1ω7c and C16 : 0. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids. The major isoprenoid quinone was Q-10. The DNA G+C content was 64.6 mol%. The DNA-DNA hybridization value for gyp-1T with P. lutimaris HDM-25T and P. aminovorans DM-82T was less than 50 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that gyp-1T should be classified as representing a novel species of the genus Paracoccus, for which the name Paracoccus mangrovi sp. nov. is presented. The type strain is gyp-1T (=BCRC 80920T=LMG 29172T=KCTC 42899T).
从台湾的红树林中分离出一株名为gyp-1T的细菌菌株，并采用多相分类法对其进行了表征。gyp-1T细胞革兰氏染色阴性，好氧，能积累聚-β-羟基丁酸，不运动，呈球状或短杆状，形成奶油色菌落。在15-37°C（最适温度为25-30°C）、pH 5.5-7.0（最适pH为6.0）和0-4% NaCl（最适浓度为1-2%）的条件下可生长。基于16S rRNA基因序列的系统发育分析表明，gyp-1T代表副球菌属的一个成员，与卢氏副球菌HDM-25T（97.8%）和氨基副球菌DM-82T（97.7%）的序列相似性最高。gyp-1T的主要脂肪酸（>10%）为C18:1ω7c和C16:0。极性脂质谱由磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、二磷脂酰甘油、一种未鉴定的糖脂和两种未鉴定的磷脂组成。主要类异戊二烯醌为Q-10。DNA G+C含量为64.6 mol%。gyp-1T与卢氏副球菌HDM-25T和氨基副球菌DM-82T的DNA-DNA杂交值小于50%。不同的表型特征以及系统发育推断表明，gyp-1T应被归类为副球菌属的一个新物种，为此提出了红树林副球菌新种的名称。模式菌株为gyp-1T（=BCRC 80920T=LMG 29172T=KCTC 42899T）。
