Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USA.
J Microbiol Biotechnol. 2024 Oct 28;34(10):2012-2022. doi: 10.4014/jmb.2404.04053. Epub 2024 Aug 9.
In this study, a novel species within the genus was isolated from the coastal soil of Dokdo (Seodo) Island and investigated. We elucidated the novel species, designated MBLB3053, through genomic analysis of novel functional microbial resources. Cells were gram-negative, non-motile, and coccoid, and the colony was light orange in color. Phylogenetic analysis based on the 16S rRNA gene showed that strain MBLB3053 was related to the genus , with 98.5% similarity to . Comparative genome analysis also revealed the strain to be a novel species of the genus by average nucleotide identity and in silico DNA-DNA hybridization values. Through secondary metabolite analysis, terpene biosynthetic gene clusters associated with carotenoid biosynthesis were found in strain MBLB3053. Using high-performance liquid chromatography, strain MBLB3053 was confirmed to produce carotenoids, including all--astaxanthin, by comparison to the standard compound. Notably, the isolate was also confirmed to produce carotenoids that other closely related species did not produce. Based on this comprehensive polyphasic taxonomy, strain MBLB3053 represents a novel species within the genus , for which the name sp. nov is proposed. The type strain was MBL3053 (=KCTC 8269 =JCM 36634). These findings support the research and resource value of this novel species, which was isolated from the Dokdo environmental microbiome.
在这项研究中，从独岛（东岛）沿海土壤中分离并研究了一个属于的新物种。我们通过对新型功能微生物资源的基因组分析，阐明了这个新物种，命名为 MBLB3053。细胞呈革兰氏阴性，非运动性，球形，菌落颜色呈浅橙色。基于 16S rRNA 基因的系统发育分析表明，MBLB3053 菌株与属内的其他种具有 98.5%的相似性，与最相似。比较基因组分析也表明，该菌株通过平均核苷酸同一性和体外 DNA-DNA 杂交值，属于的一个新型物种。通过次生代谢产物分析，在 MBLB3053 菌株中发现了与类胡萝卜素生物合成相关的萜烯生物合成基因簇。通过高效液相色谱法，通过与标准化合物进行比较，证实 MBLB3053 菌株产生包括全-虾青素在内的类胡萝卜素。值得注意的是，该分离株还被证实产生了其他密切相关的物种没有产生的类胡萝卜素。基于这种全面的多相分类学，MBLB3053 菌株代表了属内的一个新物种，因此提出了 sp. nov 的名称。该模式菌株为 MBL3053(=KCTC 8269=JCM 36634)。这些发现支持了对这个从独岛环境微生物组中分离出来的新型物种的研究和资源价值。
