Huang Jing, Li Jiahong, Cao Min, Liao Shuijiao, Wang Gejiao
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
State Key Laboratory of Agricultural Microbiology, College of Basic Science, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2646-2652. doi: 10.1099/ijsem.0.002004. Epub 2017 Aug 15.
A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8 % similarity), Blastococcus saxobsidens BC444T (93.1 %) and Geodermatophilus aquaeductus BMG801T (92.8 %). It contained iso-C16 : 0, iso-C15 : 0, C17 : 1ω6c, iso-C14 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) as the major fatty acids (>5 %), with MK-9(H4), MK-8(H4) and MK-9(H6) as the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36T showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for α-glucosidase and having C17 : 1ω6c and iso-C14 : 0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36T represents a novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans gen. nov., sp. nov. is proposed. The type strain of 'Cumulibacter manganitolerans' is 2-36T (=CCTCC AA 2016026T=DSM 103787T).
从锰矿污水排放口的污泥中分离出一株革兰氏阳性放线菌，命名为菌株2-36T。对16S rRNA基因进行系统发育分析，结果表明菌株2-36T在嗜皮菌科中自成一个分支，与玫瑰适度杆菌KLBMP 1279T（相似度93.8%）、石栖芽球菌BC444T（93.1%）和沟渠嗜皮菌BMG801T（92.8%）亲缘关系密切。其主要脂肪酸（>5%）包括异-C16 : 0、异-C15 : 0、C17 : 1ω6c、异-C14 : 0以及特征性总和3（C16 : 1ω6c和/或C16 : 1ω7c），醌类为MK-9(H4)、MK-8(H4)和MK-9(H6)。细胞壁含有内消旋二氨基庚二酸作为诊断性二氨基酸，DNA的G+C含量为70.1 mol%。极性脂包括双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未知糖脂、一种未知氨基磷脂和两种未知磷脂。与亲缘关系密切的菌株相比，菌株2-36T表现出一些独特特征，如独特的系统发育分支、苯乙酸同化试验呈阳性、α-葡萄糖苷酶试验呈阴性以及以C17 : 1ω6c和异-C14 : 0作为主要脂肪酸。基于多相分析，菌株2-36T代表嗜皮菌科内一个新属的一个新物种，为此提出新属名为Cumulibacter manganitolerans gen. nov.，新种名为Cumulibacter manganitolerans sp. nov.。“耐锰Cumulibacter”的模式菌株为2-36T（=CCTCC AA 2016026T=DSM 103787T）。
