Laser Analytics Group, Department of Chemical Engineering and Biotechnology, Cambridge University, Pembroke Street, Cambridge, CB2 3RA, UK.
Methods Appl Fluoresc. 2016 Jun 27;4(2):022004. doi: 10.1088/2050-6120/4/2/022004.
For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.
二十多年来，单分子光谱学一直在为我们提供关于分子水平的自然的宝贵见解。大约 10 年前，该技术发展成为超分辨率成像方法，为该领域带来了强大的推动力，克服了光学衍射带来的限制。如今，单分子超分辨率成像已被常规用于研究细胞中大分子的功能和结构。与此同时，还开发了计算方法，可以提供纳米级分子数量和位置的信息。在本综述中，我们概述了从单分子光谱学发展到定位显微镜技术的技术发展。然后，我们对该技术在神经科学研究领域的应用进行了全面回顾。
