Butler-Hallissey Ciarán, Leterrier Christophe
Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005, Marseille, France.
Npj Imaging. 2024 Dec 4;2(1):50. doi: 10.1038/s44303-024-00054-y.
The complexity of the brain organization and the unique architecture of neurons have motivated neuroscientists to stay at the forefront of cellular microscopy and rapidly take advantage of technical developments in this field. Among these developments, super-resolution microscopy has transformed our understanding of neurobiology by allowing us to image identified macromolecular scaffolds and complexes directly in cells. Super-resolution microscopy approaches have thus provided key insights into the organization and functions of the neuronal cytoskeleton and its unique nanostructures. These insights are the focus of our review, where we attempt to provide a panorama of super-resolution microscopy applications to the study of the neuronal cytoskeleton, delineating the progress they have made possible and the current challenges they meet.
大脑组织的复杂性和神经元独特的结构促使神经科学家站在细胞显微镜技术的前沿，并迅速利用该领域的技术发展。在这些发展中，超分辨率显微镜通过使我们能够直接在细胞中对已识别的大分子支架和复合物进行成像，改变了我们对神经生物学的理解。因此，超分辨率显微镜方法为神经元细胞骨架的组织和功能及其独特的纳米结构提供了关键见解。这些见解是我们综述的重点，我们试图全景式地介绍超分辨率显微镜在神经元细胞骨架研究中的应用，阐述它们所取得的进展以及当前面临的挑战。
