Department of Chemistry, University of Patras, 26500 Patras, Greece; Department of Oenology and Beverage Technology, Eastern Macedonia and Thrace Institute of Technology, 661 00 Kavala, Greece.
Department of Chemistry, University of Patras, 26500 Patras, Greece.
Bioresour Technol. 2017 Nov;244(Pt 1):629-634. doi: 10.1016/j.biortech.2017.07.163. Epub 2017 Jul 29.
Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30°C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension. This positive effect was also shown when low concentration mixed agro-industrial waste suspensions were used as substrates, leading to 85% enhanced SCO production in comparison with free cells. Higher fatty acid (HFA) analysis showed that yeast immobilisation led to increased formation of unsaturated HFAs (6%) and reduced saturated HFAs (5%) compared to free cells.
报道了利用固定化在去木质素多孔纤维素上的产油酵母 Lipomyces starkeyi DSM 70296 来提高单细胞油（SCO）的产量。最初使用了纯葡萄糖培养基。评估了底物 pH 值和处理温度的影响，结果表明 30°C 和 pH 5.0 是固定化酵母生产 SCO 的最佳条件。与悬浮的游离细胞相比，固定化技术分别使葡萄糖培养基中的脂质积累和细胞生长增加了 44%和 8%。当使用低浓度混合农业工业废物悬浮液作为底物时，也表现出这种积极的效果，与游离细胞相比，SCO 的产量提高了 85%。高级脂肪酸（HFA）分析表明，与游离细胞相比，酵母固定化导致不饱和 HFA 的形成增加了 6%，饱和 HFA 减少了 5%。
