Integrated Cancer Research Center, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA, 30332, USA.
School of Biological Sciences, and Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA, 30332, USA.
Sci Rep. 2017 Aug 15;7(1):8171. doi: 10.1038/s41598-017-08502-z.
High-throughput technologies have identified significant changes in patterns of mRNA expression over cancer development but the functional significance of these changes often rests upon the assumption that observed changes in levels of mRNA accurately reflect changes in levels of their encoded proteins. We systematically compared the expression of 4436 genes on the RNA and protein levels between discrete tumor samples collected from the ovary and from the omentum of the same OC patient. The overall correlation between global changes in levels of mRNA and their encoding proteins is low (r = 0.38). The majority of differences are on the protein level with no corresponding change on the mRNA level. Indirect and direct evidence indicates that a significant fraction of the differences may be mediated by microRNAs.
高通量技术已经确定了在癌症发展过程中 mRNA 表达模式的显著变化，但这些变化的功能意义通常取决于这样一个假设，即观察到的 mRNA 水平的变化准确反映了其编码蛋白水平的变化。我们系统地比较了来自卵巢和同一 OC 患者网膜的离散肿瘤样本中 4436 个基因在 RNA 和蛋白质水平上的表达。mRNA 和其编码蛋白水平整体变化之间的相关性较低（r=0.38）。大多数差异出现在蛋白质水平上，而 mRNA 水平上没有相应的变化。间接和直接证据表明，很大一部分差异可能是由 microRNAs 介导的。
