Shi Yi, Niu Kuiju, Huang Bingru, Liu Wenhui, Ma Huiling
College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China.
Key Laboratory of Grassland Ecosystems, The Ministry of Education of China, Lanzhou 730070, China.
Molecules. 2017 Aug 16;22(8):1318. doi: 10.3390/molecules22081318.
Bacterial volatile compounds (BVCs) have been reported to enhance plant growth and elicit plant defenses against fungal infection and insect damage. The objective of this study was to determine transcriptomic changes in response to synthetic BVC that could be associated with plant resistance to in creeping bentgrass. The 2,3-butanediol (BD) (250 µM) was sprayed on creeping bentgrass leaves grown in jam jars. The result showed that synthetic BD induced plant defense against for creeping bentgrass. Transcriptomic analysis demonstrated that more genes were repressed by BD while less showed up-regulation. BD suppressed the expression of some regular stress-related genes in creeping bentgrass, such as pheromone activity, calcium channel activity, photosystem II oxygen evolving complex, and hydrolase activity, while up-regulated defense related transcription factors (TFs), such as basic helix-loop-helix (bHLH) TFs, cysteine2-cysteine2-contans-like (C2C2-CO) and no apical meristem TFs (NAC). Other genes related to disease resistance, such as jasmonic acid (JA) signaling, leucine rich repeats (LRR)-transmembrane protein kinase, gene 5 receptor kinase and nucleotide binding site-leucine rich repeats (NBS-LRR) domain containing plant resistance gene (-gene) were also significantly up-regulated. These results suggest that BD may induce changes to the plant transcriptome in induced systemic resistance (ISR) pathways.
据报道，细菌挥发性化合物（BVCs）可促进植物生长，并引发植物对真菌感染和昆虫损害的防御反应。本研究的目的是确定匍匐翦股颖对合成BVC产生的转录组变化，这些变化可能与植物抗性相关。将2,3-丁二醇（BD）（250μM）喷洒在果酱瓶中生长的匍匐翦股颖叶片上。结果表明，合成BD诱导了匍匐翦股颖对[此处原文缺失某种病原体名称]的植物防御反应。转录组分析表明，受BD抑制的基因更多，而上调的基因较少。BD抑制了匍匐翦股颖中一些常规的胁迫相关基因的表达，如信息素活性、钙通道活性、光系统II放氧复合体和水解酶活性，同时上调了防御相关转录因子（TFs），如碱性螺旋-环-螺旋（bHLH）转录因子、半胱氨酸2-半胱氨酸2-类似锌指蛋白（C2C2-CO）和无顶端分生组织转录因子（NAC）。其他与抗病性相关的基因，如茉莉酸（JA）信号传导、富含亮氨酸重复序列（LRR）的跨膜蛋白激酶、基因5受体激酶和含有植物抗性基因（抗病基因）的核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）结构域也显著上调。这些结果表明，BD可能在诱导系统抗性（ISR）途径中诱导植物转录组发生变化。
