Xie Linyan, Liu Lufeng, Luo Yanju, Rao Xibing, Di Yining, Liu Han, Qian Zhenfeng, Shen Qingqing, He Lilian, Li Fusheng
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
College of Resources and Environment, Yunnan Agricultural University, Kunming, China.
Front Microbiol. 2023 Jun 2;14:1180474. doi: 10.3389/fmicb.2023.1180474. eCollection 2023.
Sugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by . YC89 was isolated from sugarcane leaves and can significantly inhibited red rot disease caused by .
In this study, the genome of YC89 strain was sequenced, its genome structure and function were analyzed using various bioinformatics software, and its genome was compared with those of other homologous strains. In addition, the effectiveness of YC89 against sugarcane red rot and the evaluation of sugarcane plant growth promotion were also investigated by pot experiments.
Here, we present the complete genome sequence of YC89, which consists of a 3.95 Mb circular chromosome with an average GC content of 46.62%. The phylogenetic tree indicated that YC89 is closely related to GS-1. Comparative genome analysis of YC89 with other published strains ( FZB42, CC09, SQR9, GS-1, and DSM7) revealed that the strains had a part common coding sequences (CDS) in whereas 42 coding were unique of strain YC89. Whole-genome sequencing revealed 547 carbohydrate-active enzymes and identified 12 gene clusters encoding secondary metabolites. Additionally, functional analysis of the genome revealed numerous gene/gene clusters involved in plant growth promotion, antibiotic resistance, and resistance inducer synthesis. pot tests indicated that YC89 strain controlled sugarcane red rot and promoted the growth of sugarcane plants. Additionally, it increased the activity of enzymes involved in plant defense, such as superoxide dismutase, peroxidase, polyphenol oxidase, chitinase, and β-1,3-glucanase.
These findings will be helpful for further studies on the mechanisms of plant growth promotion and biocontrol by and provide an effective strategy for controlling red rot in sugarcane plants.
甘蔗是全球最重要的糖料作物之一，然而，甘蔗生产受到甘蔗赤腐病的严重限制，这是一种由[病原菌名称缺失]引起的土传病害。YC89从甘蔗叶片中分离得到，能够显著抑制由[病原菌名称缺失]引起的赤腐病。
在本研究中，对YC89菌株的基因组进行了测序，使用各种生物信息学软件分析其基因组结构和功能，并将其基因组与其他同源菌株的基因组进行比较。此外，还通过盆栽试验研究了YC89对甘蔗赤腐病的防治效果以及对甘蔗植株生长促进作用的评价。
在此，我们展示了YC89的完整基因组序列，其由一条3.95 Mb的环状染色体组成，平均GC含量为46.62%。系统发育树表明YC89与[菌株名称缺失]GS-1密切相关。对YC89与其他已发表菌株（[菌株名称缺失]FZB42、[菌株名称缺失]CC09、[菌株名称缺失]SQR9、[菌株名称缺失]GS-1和[菌株名称缺失]DSM7）的比较基因组分析显示，这些菌株在[相关区域名称缺失]中有部分共同编码序列（CDS），而42个编码序列是YC89菌株特有的。全基因组测序揭示了547种碳水化合物活性酶，并鉴定出12个编码次级代谢产物的基因簇。此外，基因组功能分析揭示了许多参与植物生长促进、抗生素抗性和抗性诱导物合成的基因/基因簇。盆栽试验表明，YC89菌株能防治甘蔗赤腐病并促进甘蔗植株生长。此外，它还提高了参与植物防御的酶的活性，如超氧化物歧化酶、过氧化物酶、多酚氧化酶、几丁质酶和β-1,3-葡聚糖酶。
这些发现将有助于进一步研究[菌株名称缺失]促进植物生长和生物防治的机制，并为控制甘蔗植株中的赤腐病提供有效策略。
