a Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
b School of Biosciences and Biotechnology, Faculty of Science, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia.
Genome. 2017 Dec;60(12):1045-1050. doi: 10.1139/gen-2017-0100. Epub 2017 Aug 16.
While it is crucial for developing countries like Malaysia to achieve self-sufficiency in rice (Oryza sativa L.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. The present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. A marker-assisted backcross (MABC) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding Malaysian varieties, MR84 and MR219, within two years utilising less than 50 molecular markers. Coupled with phenotypic screening, one single foreground marker (fgr-SNP) and 48 background markers were selected and utilised, revealing recovery of at least 90% of recurrent parent genome (RPG) in merely two backcross generations. Collectively, the yield potential of the developed BCF lines (BLs) was higher (P > 0.05) than the donor parent, MRQ74, and similar (P < 0.05) to both the recurrent parents, MR84 and MR219. In addition, some of the developed BLs showed good grain quality, such as having long grain. We believe that this is the first report comprising the validation and utilisation of the single functional marker system (fgr-SNP) in introgressing the fgr gene into different rice varieties.
虽然马来西亚等发展中国家实现水稻（Oryza sativa L.）自给自足至关重要，但能够生产出高质量的大米，特别是香米，也同样至关重要，而香米的需求往往需要通过进口来满足。本研究旨在以及时和具有成本效益的方式开发高产香稻。采用标记辅助回交（MABC）方法，在两年内利用不到 50 个分子标记，将香味基因（fgr）导入两个高产的马来西亚品种 MR84 和 MR219 中。结合表型筛选，选择和利用了一个单一的前景标记（fgr-SNP）和 48 个背景标记，结果显示仅在两代回交中就恢复了至少 90%的轮回亲本基因组（RPG）。总体而言，所开发的 BCF 系（BL）的产量潜力（P>0.05）高于供体亲本 MRQ74，与轮回亲本 MR84 和 MR219 相似（P<0.05）。此外，一些开发的 BL 表现出良好的谷物品质，例如长粒。我们相信，这是第一个报告，包含了单功能标记系统（fgr-SNP）在将 fgr 基因导入不同水稻品种中的验证和利用。
