Kumar Ashish, Maurya Antim K, Chand Gopi, Agnihotri Vijai K
a Academy of Scientific and Innovative Research , CSIR- Institute of Himalayan Bioresource Technology , Palampur , India.
b Natural Product Chemistry and Process Development Division , CSIR-Institute of Himalayan Bioresource Technology , Palampur , India.
Nat Prod Res. 2018 Apr;32(7):826-833. doi: 10.1080/14786419.2017.1365069. Epub 2017 Aug 17.
Costus speciosus had been used in oriental systems of medicines, to treat diverse ailments. The present study was focused on NMR, GC-MS and UPLC/ESI-MS/MS-based metabolic profiling of C. speciosus. This metabolic study resulted in the identification of 91 and quantification of 69 metabolites. Caffeic acid derivatives previously unreported in C. speciosus were also identified. High quantity of steroidal saponins namely methyl protogracillin (297.97 ± 0.07 mg/g dried wt.) and dioscin (158.72 ± 0.27 mg/g dried wt.) were observed in butanol fraction of rhizomes. Health care metabolites including caffeic acid (37.88 ± 0.04 mg/g dried wt.) and trehalose (75.12 ± 0.08 mg/g dried wt.) were also detected in ethyl acetate and aqueous fractions of rhizomes, respectively. Metabolites of nutraceutical and biological significance including eremanthine (5.14 ± 0.68%, peak area), tocopherols (22%), sterols (25%) were also identified from hexane fractions of rhizomes and leaves using GC-MS. The analytical techniques used had successfully differentiated metabolites composition among leaves and rhizomes.
闭鞘姜已被用于东方医学体系中治疗多种疾病。本研究聚焦于基于核磁共振（NMR）、气相色谱 - 质谱联用（GC - MS）和超高效液相色谱 - 电喷雾串联质谱（UPLC/ESI - MS/MS）的闭鞘姜代谢谱分析。这项代谢研究鉴定出了91种代谢物，并对69种代谢物进行了定量分析。还鉴定出了闭鞘姜中先前未报道的咖啡酸衍生物。在根茎的丁醇部位观察到大量的甾体皂苷，即原人参二醇甲酯（297.97 ± 0.07毫克/克干重）和薯蓣皂苷元（158.72 ± 0.27毫克/克干重）。在根茎的乙酸乙酯部位和水部位分别检测到了具有保健作用的代谢物，即咖啡酸（37.88 ± 0.04毫克/克干重）和海藻糖（75.12 ± 0.08毫克/克干重）。使用气相色谱 - 质谱联用（GC - MS）从根茎和叶子的己烷部位还鉴定出了具有营养保健和生物学意义的代谢物，包括艾里曼辛（5.14 ± 0.68%，峰面积）、生育酚（约22%）、甾醇（约25%）。所使用的分析技术成功地区分了叶子和根茎之间的代谢物组成。
