Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia.
Phytochemistry. 2011 Jan;72(1):126-35. doi: 10.1016/j.phytochem.2010.10.013. Epub 2010 Nov 19.
The occurrence of steroidal saponins in the rhizomes of Yucca gloriosa has been detected by LC-MS. On the basis of the LC-MS analysis, five steroidal glycosides, including three spirostane, one furostane and one cholestane glycosides, along with seven known compounds have been isolated and characterized by ESI-MS and by the extensive use of 1D- and 2D-NMR experiments. Quantitative analysis of the steroidal glycosides in Y. gloriosa rhizomes was performed by an LC-MS method validated according to European Medicines Agency (EMEA) guidelines. The dried BuOH extract obtained from rhizomes contains more than 25% w/w of glycosides, thus Y. gloriosa rhizomes can be considered a rich source of steroidal glycosides.
通过 LC-MS 检测到丝兰根茎中甾体皂苷的存在。根据 LC-MS 分析，分离并通过 ESI-MS 和广泛使用 1D 和 2D-NMR 实验鉴定出五种甾体糖苷，包括三种螺甾烷、一种呋甾烷和一种胆甾烷糖苷，以及七种已知化合物。根据欧洲药品管理局 (EMEA) 指南验证的 LC-MS 方法对丝兰根茎中的甾体糖苷进行定量分析。从根茎中获得的干燥 BuOH 提取物含有超过 25%w/w 的糖苷，因此丝兰根茎可以被认为是甾体糖苷的丰富来源。
