Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, Italy.
The BioRobotics Institute, Scuola Superiore Sant'Anna, Viale Rinaldo Piaggio 34, 56025, Pontedera, Italy.
Macromol Biosci. 2017 Nov;17(11). doi: 10.1002/mabi.201700128. Epub 2017 Aug 16.
The development of smart biointerfaces combining multiple functions is crucial for triggering a variety of cellular responses. In this work, wrinkled organic interfaces based on the conducting polymer poly(3,4-ethylene dioxythiophene) doped with poly(styrene sulfonate) are developed with the aim to simultaneously convey electrical and topographical stimuli to cultured cells. The surface wrinkling of thin films on heat-shrink polymer sheets allows for rapid patterning of self-assembled anisotropic topographies characterized by micro/sub-microscale aligned wrinkles. The developed interfaces prove to support the growth and differentiation of neural cells (SH-SY5Y, human neuroblastoma) and are remarkably effective in promoting axonal guidance, by guiding and stimulating the neurite growth in differentiating cells. Electrical stimulation with biphasic pulses delivered through the conductive wrinkled interface is found to further promote the neurite growth, demonstrating the suitability of such interfaces as platforms for conveying multiple stimuli to cells and tissues.
智能生物界面的发展结合了多种功能，对于触发各种细胞反应至关重要。在这项工作中，基于掺杂聚（苯乙烯磺酸盐）的导电聚合物聚（3,4-亚乙基二氧噻吩）开发了具有褶皱的有机界面，旨在向培养细胞同时传递电和形貌刺激。在热缩聚合物片上的薄膜的表面褶皱允许快速图案化自组装各向异性形貌，其特征在于微/亚微尺度的定向褶皱。所开发的界面被证明可以支持（SH-SY5Y，人神经母细胞瘤）神经细胞的生长和分化，并且在促进轴突导向方面非常有效，通过引导和刺激分化细胞中的神经突生长。通过导电褶皱界面传输双相脉冲的电刺激被发现进一步促进了神经突的生长，证明了这种界面作为向细胞和组织传递多种刺激的平台的适用性。
