Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Da'an Dist., Taipei City 10607, Taiwan.
Department of Chemical Engineering, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2354-2362. doi: 10.1021/acsabm.0c01204. Epub 2021 Feb 23.
Although conductive bioelectronic interfaces (BEIs) can allow neural cell culturing while providing electrical stimulation (ES) to the nervous system, there are few simple approaches for the preparation of conductive BEIs with topographical features designed for cell manipulation. In this study, we developed a facile method for fabricating microwrinkled poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) films through spin-coating onto pre-elongated polydimethylsiloxane substrates. The microwrinkles of our PEDOT:PSS films pre-elongated by 20 and 40% had average widths of 6.47 ± 1.49 and 5.39 ± 1.53 μm, respectively. These microwrinkled PEDOT:PSS films promoted the directional ordering of neurite outgrowth of PC12 cells and displayed favorable biocompatibility and outstanding electrochemical properties for long-term ES treatment. When using this BEI platform, the level of PC12 gene expression of Neun was enhanced significantly after 5 days of culturing in differentiation media and under ES, in line with the decreased expression of early phase markers. Therefore, such readily fabricated microwrinkled PEDOT:PSS films are promising candidates for use as BEIs for tissue regenerative medicine.
虽然导电生物电子接口 (BEI) 可以在为神经系统提供电刺激 (ES) 的同时允许神经细胞培养，但很少有简单的方法可以制备具有用于细胞操作的形貌特征的导电 BEI。在这项研究中，我们通过旋涂到预先拉伸的聚二甲基硅氧烷 (PDMS) 基底上，开发了一种简便的方法来制造具有微皱纹的聚(3,4-亚乙基二氧噻吩):聚苯乙烯磺酸盐 (PEDOT:PSS) 薄膜。我们的 PEDOT:PSS 薄膜在预拉伸 20%和 40%时的微皱纹平均宽度分别为 6.47 ± 1.49 μm 和 5.39 ± 1.53 μm。这些微皱纹的 PEDOT:PSS 薄膜促进了 PC12 细胞突起的定向生长，并表现出良好的生物相容性和出色的电化学性能，可用于长期 ES 治疗。当使用这种 BEI 平台时，在分化培养基中培养 5 天后，在 ES 下，PC12 基因表达的 Neun 水平显著增强，与早期标志物的表达降低一致。因此，这种易于制备的微皱纹 PEDOT:PSS 薄膜有望成为组织再生医学中使用的 BEI 的候选材料。
