Department of Biology, Alexandru Ioan Cuza University of Iasi, Iasi, Romania.
Pôle Recherche Innovation et Entrepreneuriat (PRIE), Institut Universitaire de la Côte, Douala, Cameroon.
J Cell Mol Med. 2018 Jan;22(1):111-122. doi: 10.1111/jcmm.13299. Epub 2017 Aug 16.
We investigated the neuropharmacological effects of the methanolic extract from Lactuca capensis Thunb. leaves (100 and 200 mg/kg) for 21 days on memory impairment in an Alzheimer's disease (AD) rat model produced by direct intraventricular delivery of amyloid-β1-42 (Aβ1-42). Behavioural assays such as Y-maze and radial arm maze test were used for assessing memory performance. Aβ1-42 decreased cognitive performance in the behavioural tests which were ameliorated by pre-treatment with the methanolic extract. Acetylcholinesterase activity and oxidant-antioxidant balance in the rat hippocampus were abnormally altered by Aβ1-42 treatment while these deficits were recovered by pre-treatment with the methanolic extract. In addition, rats were given Aβ1-42 exhibited in the hippocampus decreased brain-derived neurotrophic factor (BDNF) mRNA copy number and increased IL-1β mRNA copy number which was reversed by the methanolic extract administration. These findings suggest that the methanolic extract could be a potent neuropharmacological agent against dementia via modulating cholinergic activity, increasing of BDNF levels and promoting antioxidant action in the rat hippocampus.
我们研究了菊苣叶甲醇提取物（100 和 200 mg/kg）对通过脑室直接给予淀粉样蛋白-β1-42（Aβ1-42）产生的阿尔茨海默病（AD）大鼠模型记忆障碍的神经药理学作用，为期 21 天。行为测定，如 Y 迷宫和放射臂迷宫测试，用于评估记忆性能。Aβ1-42 降低了行为测试中的认知表现，而用甲醇提取物预处理可改善这种情况。Aβ1-42 处理异常改变了大鼠海马中的乙酰胆碱酯酶活性和氧化还原平衡，而甲醇提取物预处理可恢复这些缺陷。此外，给予 Aβ1-42 的大鼠在海马体中表现出脑源性神经营养因子（BDNF）mRNA 拷贝数减少和 IL-1β mRNA 拷贝数增加，而甲醇提取物的给药可逆转这种情况。这些发现表明，甲醇提取物可能通过调节胆碱能活性、增加 BDNF 水平和促进大鼠海马体中的抗氧化作用，成为一种有效的神经药理学药物，可用于治疗痴呆。
