Oliveira Gabriela Cristina de, Tereza Guida Paola Genovez, Boteon Ana Paula, Ferrairo Brunna Mota, Gonçalves Priscilla Santana Pinto, Silva Thiago Cruvinel da, Honório Heitor Marques, Rios Daniela
Department of Pediatric Dentistry, Orthodontics and Public Health Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Department of Operative Dentistry, Endodontics and Dental Materials Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
PLoS One. 2017 Aug 17;12(8):e0182347. doi: 10.1371/journal.pone.0182347. eCollection 2017.
This in vitro study evaluated the impact of initial erosion on the susceptibility of enamel to further erosive challenge. Thirty bovine enamel blocks were selected by surface hardness and randomized into two groups (n = 15): GC- group composed by enamel blocks without erosion lesion and GT- group composed by enamel blocks with initial erosion lesion. The baseline profile of each block was determined using the profilometer. The initial erosion was produced by immersing the blocks into HCl 0.01 M, pH 2.3 for 30 seconds, under stirring. The erosive cycling consisted of blocks immersion in hydrochloric acid (0.01 M, pH 2.3) for 2 minutes, followed by immersion in artificial saliva for 120 minutes. This procedure was repeated 4 times a day for 5 days, and the blocks were kept in artificial saliva overnight. After erosive cycling, final profile measurement was performed. Profilometry measured the enamel loss by the superposition of initial and final profiles. Data were analyzed by t-test (p<0.05). The result showed no statistically significant difference between groups (GS = 14.60±2.86 and GE = .14.69±2.21 μm). The presence of initial erosion on bovine dental enamel does not enhance its susceptibility to new erosive challenges.
这项体外研究评估了初始侵蚀对牙釉质对进一步侵蚀性挑战易感性的影响。通过表面硬度选择30个牛牙釉质块,并随机分为两组(n = 15):GC组由无侵蚀病变的牙釉质块组成,GT组由有初始侵蚀病变的牙釉质块组成。使用轮廓仪确定每个块的基线轮廓。通过在搅拌下将块浸入0.01 M、pH 2.3的HCl中30秒来产生初始侵蚀。侵蚀循环包括将块浸入盐酸(0.01 M,pH 2.3)中2分钟,然后浸入人工唾液中120分钟。该程序每天重复4次,共5天,并且块在人工唾液中过夜保存。在侵蚀循环后,进行最终轮廓测量。轮廓测定法通过叠加初始和最终轮廓来测量牙釉质损失。数据通过t检验进行分析(p<0.05)。结果显示两组之间无统计学显著差异(GS = 14.60±2.86和GE = 14.69±2.21μm)。牛牙釉质上初始侵蚀的存在不会增加其对新的侵蚀性挑战的易感性。