Chen Yelong, Zhang Ying, Tan Yang, Liu Zhaoyong
Department of Orthopaedics, First Affiliated Hospital of Shantou University Medical College, No.57 Changping Road, Shantou, Guangdong 515041, China; Department of Pathology, Shantou University Medical College, Shantou, Guangdong Province, China.
Department of Pathology, Shantou University Medical College, Shantou, Guangdong Province, China.
Biochem Biophys Res Commun. 2017 Oct 14;492(2):184-191. doi: 10.1016/j.bbrc.2017.08.043. Epub 2017 Aug 14.
Secreted protein acidic and rich in cysteine (SPARC) is matricellular protein that modulates interactions between cells and the extracellular matrix. The role of SPARC in carcinogenesis is controversialin that SPARC can be a tumor suppressor, but overexpression of SPARC is associated with poorer prognosis.
We collected 145 esophageal squamous cell carcinoma and adjacent normal tissues in Shantou, a high incidence region for esophageal cancer. The mRNA and protein expression levels of SPARC in cancer tissue and in adjacent normal mucosa were measured by qRT-PCR, western blot and immunohistochemistry (IHC).
The mRNA and protein levels of SPARCwere5.78-fold higher in cancer tissues compared with the case-matched normal epithelium. High expression levels of SPARC in ESCC parenchyma, as detected by IHC, were related to lymph node metastasis and poor prognosis (p = 0.049 and p = 0.04).
High expression of SPARC in the parenchyma may be a potential predictor of prognosis, suggesting SPARC could serve as a therapeutic target in ESCC.
富含半胱氨酸的酸性分泌蛋白（SPARC）是一种基质细胞蛋白，可调节细胞与细胞外基质之间的相互作用。SPARC在致癌过程中的作用存在争议，因为SPARC可以是一种肿瘤抑制因子，但SPARC的过表达与较差的预后相关。
我们在食管癌高发地区汕头收集了145例食管鳞状细胞癌及癌旁正常组织。采用qRT-PCR、蛋白质印迹法和免疫组织化学（IHC）检测癌组织和癌旁正常黏膜中SPARC的mRNA和蛋白质表达水平。
与病例匹配的正常上皮相比，癌组织中SPARC的mRNA和蛋白质水平高5.78倍。免疫组织化学检测显示，食管鳞状细胞癌实质中SPARC的高表达水平与淋巴结转移和预后不良相关（p = 0.049和p = 0.04）。
实质中SPARC的高表达可能是预后的潜在预测指标，提示SPARC可作为食管鳞状细胞癌的治疗靶点。
