ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, NSW 2500, Australia.
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, NSW 2500, Australia.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:386-393. doi: 10.1016/j.colsurfb.2017.07.087. Epub 2017 Aug 1.
Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants. Notwithstanding its potential, a property that can inhibit PDMS bioactivity is the high hydrophobicity, limiting its use to date in tissue engineering. Here, we describe an efficient approach to produce porous, durable and cytocompatible PDMS-based 3D structures, coated with reduced graphene oxide (RGO). The RGO/PDMS scaffold has good mechanical strength and with pore sizes ranging from 10 to 600μm. Importantly, the scaffold is able to support growth and differentiation of human adipose stem cells (ADSCs) to an osteogenic cell lineage, indicative of its potential as a transition structure of an osseointegrated implant.
在骨科手术中，骨整合对于植入物的成功起着重要作用。传统的植入物表面处理方法旨在改善骨整合，但在促进细胞生长和增殖方面的性能有限。聚二甲基硅氧烷（PDMS）是一种广泛使用的硅基有机聚合物材料，具有在化妆品、家庭应用和机械工程等领域的应用性能。此外，PDMS 的生物相容性，部分归因于其高氧溶解度，使其成为细胞植入物的理想材料。尽管具有这种潜力，但 PDMS 的高疏水性会抑制其生物活性，这限制了其在组织工程中的应用。在这里，我们描述了一种生产具有多孔、耐用和细胞相容性的 PDMS 基 3D 结构的有效方法，该结构涂有还原氧化石墨烯（RGO）。RGO/PDMS 支架具有良好的机械强度，孔径范围为 10 至 600μm。重要的是，支架能够支持人脂肪干细胞（ADSCs）向成骨细胞系的生长和分化，表明其具有作为骨整合植入物过渡结构的潜力。
