Dong Wenjun, Hou Lijuan, Li Tingting, Gong Ziqiang, Huang Huandi, Wang Ge, Chen Xiaobo, Li Xiaoyun
Center for Nanoscience and Nanotechnology, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
Sci Rep. 2015 Dec 21;5:18266. doi: 10.1038/srep18266.
Scaffold biomaterials with open pores and channels are favourable for cell growth and tissue regeneration, however the inherent poor mechanical strength and low surface activity limit their applications as load-bearing bone grafts with satisfactory osseointegration. In this study, macro-porous graphene oxide (GO) modified titanate nanowire scaffolds with desirable surface chemistry and tunable mechanical properties were prepared through a simple hydrothermal process followed by electrochemical deposition of GO nanosheets. The interconnected and porous structure of the GO/titanate nanowire scaffolds provides a large surface area for cellular attachment and migration and displays a high compressive strength of approximately 81.1 MPa and a tunable Young's modulus over the range of 12.4-41.0 GPa, which satisfies site-specific requirements for implantation. Surface chemistry of the scaffolds was modulated by the introduction of GO, which endows the scaffolds flexibility in attaching and patterning bioactive groups (such as -OH, -COOH and -NH2). In vitro cell culture tests suggest that the GO/titanate nanowire scaffolds act as a promising biomaterial candidate, in particular the one terminated with -OH groups, which demonstrates improved cell viability, and proliferation, differentiation and osteogenic activities.
具有开放孔隙和通道的支架生物材料有利于细胞生长和组织再生，然而其固有的机械强度差和表面活性低限制了它们作为具有令人满意的骨整合的承重骨移植材料的应用。在本研究中，通过简单的水热过程，随后进行氧化石墨烯（GO）纳米片的电化学沉积，制备了具有理想表面化学性质和可调机械性能的大孔氧化石墨烯修饰钛酸盐纳米线支架。GO/钛酸盐纳米线支架的相互连接的多孔结构为细胞附着和迁移提供了大表面积，并显示出约81.1MPa的高抗压强度和12.4-41.0GPa范围内的可调杨氏模量，这满足了植入的特定部位要求。通过引入GO来调节支架的表面化学性质，这赋予支架在附着和图案化生物活性基团（如-OH、-COOH和-NH2）方面的灵活性。体外细胞培养测试表明，GO/钛酸盐纳米线支架是一种有前途的生物材料候选物，特别是以-OH基团终止的支架表现出改善的细胞活力、增殖、分化和成骨活性。
