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用于表达表皮生长因子受体（EGFR）的恶性肿瘤分子成像的高亲和力重复抗体。

A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors.

作者信息

Yun Misun, Kim Dong-Yeon, Lee Joong-Jae, Kim Hyeon-Sik, Kim Hyung-Seok, Pyo Ayoung, Ryu Yiseul, Kim Tae-Yoon, Zheng Jin Hai, Yoo Su Woong, Hyun Hoon, Oh Gyungseok, Jeong Jaeho, Moon Myeongju, Min Jung-Hyun, Kwon Seong Young, Kim Jung Young, Chung Euiheon, Hong Yeongjin, Lee Wansik, Kim Hak-Sung, Min Jung-Joon

机构信息

Department of Nuclear Medicine, Chonnam National University Medical School and Hwasun Hospital, Jeonnam, Republic of Korea.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.

出版信息

Theranostics. 2017 Jun 25;7(10):2620-2633. doi: 10.7150/thno.18096. eCollection 2017.

DOI:10.7150/thno.18096
PMID:28819451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5558557/
Abstract

The accurate detection of disease-related biomarkers is crucial for the early diagnosis and management of disease in personalized medicine. Here, we present a molecular imaging of human epidermal growth factor receptor (EGFR)-expressing malignant tumors using an EGFR-specific repebody composed of leucine-rich repeat (LRR) modules. The repebody was labeled with either a fluorescent dye or radioisotope, and used for imaging of EGFR-expressing malignant tumors using an optical method and positron emission tomography. Our approach enabled visualization of the status of EGFR expression, allowing quantitative evaluation in whole tumors, which correlated well with the EGFR expression levels in mouse or patients-derived colon cancers. The present approach can be effectively used for the accurate detection of EGFR-expressing cancers, assisting in the development of a tool for detecting other disease biomarkers.

摘要

在个性化医疗中，准确检测疾病相关生物标志物对于疾病的早期诊断和管理至关重要。在此，我们使用由富含亮氨酸重复序列（LRR）模块组成的EGFR特异性重复体，对表达人表皮生长因子受体（EGFR）的恶性肿瘤进行分子成像。该重复体用荧光染料或放射性同位素标记，并用于通过光学方法和正电子发射断层扫描对表达EGFR的恶性肿瘤进行成像。我们的方法能够可视化EGFR表达状态，实现对整个肿瘤的定量评估，这与小鼠或患者来源的结肠癌中的EGFR表达水平高度相关。本方法可有效用于准确检测表达EGFR的癌症，有助于开发检测其他疾病生物标志物的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b4/5558557/6ade7a8bb259/thnov07p2620g008.jpg
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