Abraham Wolf-Rainer, de Carvalho Maira Peres, da Costa Neves Thais Souto Paula, Memoria Marina Torquato, Tartuci Iago Toledo, Vancanneyt Marc, Smit John, Rohde Manfred
Helmholtz Centre for Infection Research, Chemical Microbiology, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
BCCM/LMG Bacteria Collection, Universiteit Gent, K.L. Ledeganckstraat 35, Gent, Belgium.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2804-2810. doi: 10.1099/ijsem.0.002024. Epub 2017 Aug 18.
Two Gram-negative, heterotrophic, aerobic, prosthecated, marine bacteria, designated strains MCS23T and MCS27T, were isolated from seawater samples. NaCl was required for growth. The major polar lipid detected in strain MCS27T was phosphatidylglycerol, whereas those detected in MCS23T were phosphatidylglycerol, sulfoquinovosyl diacylglycerol and 1,2-diacyl-3-α-d-glucuronopyranosyl-sn-glycerol taurineamide. The most abundant cellular fatty acids were C18 : 1ω7 and C16 : 0, hydroxyl-fatty acids were 3-OH C12 : 0 in both strains and 3-OH C11 : 0 in MCS23T. Strains MCS23T and MCS27T had DNA G+C contents of 57.0 and 55.0 mol%, respectively. The two strains shared 99.3 % 16S rRNA gene sequence similarity; levels of similarity with the type strains of species of the genus Henriciella were 99.4-97.8 % but DNA-DNA hybridizations were 53 % or lower. Besides their 16S rRNA gene sequences, the novel strains can be differentiated from other species of the genus Henriciella by cell morphology, lipid and fatty acid patterns and enzyme activities. The data obtained led to the identification of two novel species, for which the names Henriciella barbarensis sp. nov. (type strain MCS23T=LMG 28705T=CCUG 66934T) and Henriciella algicola sp. nov. (type strain MCS27T=LMG 29152T=CCUG 67844T) are proposed. As these two novel species are the first prosthecate species in the genus Henriciella, an emended genus description is also provided.
从海水样本中分离出两株革兰氏阴性、异养、需氧、具柄的海洋细菌，分别命名为菌株MCS23T和MCS27T。生长需要氯化钠。菌株MCS27T中检测到的主要极性脂是磷脂酰甘油，而在MCS23T中检测到的是磷脂酰甘油、磺基喹诺糖基二酰基甘油和1,2-二酰基-3-α-D-葡糖醛酸吡喃糖基-sn-甘油牛磺酸酰胺。最丰富的细胞脂肪酸是C18 : 1ω7和C16 : 0，两株菌中的羟基脂肪酸均为3-OH C12 : 0，MCS23T中还有3-OH C11 : 0。菌株MCS23T和MCS27T的DNA G+C含量分别为57.0和55.0 mol%。这两株菌的16S rRNA基因序列相似性为99.3 %；与亨氏菌属各物种模式菌株的相似性水平为99.4-97.8 %，但DNA-DNA杂交率为53 %或更低。除了16S rRNA基因序列外，这些新菌株还可通过细胞形态、脂质和脂肪酸模式以及酶活性与亨氏菌属的其他物种区分开来。所获得的数据导致鉴定出两个新物种，提议将其命名为巴氏亨氏菌（Henriciella barbarensis sp. nov.）（模式菌株MCS23T=LMG 28705T=CCUG 66934T）和嗜藻亨氏菌（Henriciella algicola sp. nov.）（模式菌株MCS27T=LMG 29152T=CCUG 67844T）。由于这两个新物种是亨氏菌属中首批具柄的物种，因此还提供了该属的修订描述。
