Ren Wen-Ting, Cheng Hong, Wang Chun-Sheng, Xu Xue-Wei, Zhou Peng, Wu Yue-Hong
Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
School of Oceanography, Shanghai Jiao Tong University, Shanghai 200030, PR China.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004889.
Strains M65, M69 and JN25 were isolated from seawater of the West Pacific Ocean. Cells of the three strains were Gram-stain-negative, aerobic and rod-shaped. Cells were motile by means of flagella. On the basis of the results of 16S rRNA gene sequence analysis, strains M65, M69 and JN25 showed the highest 16S rRNA gene sequence similarity to MCS27 (98.8 %), followed by DSM 19595 (98.4 %), MCS23 (98.4 %), LA220 (98.3 %), P38 (98.1 %) and SD10 (97.8 %). The 16S rRNA gene sequence similarities among the isolates were 100 %. Phylogenetic analyses revealed that the isolates fell within a cluster comprising the species and represented an independent lineage. The average nucleotide identity and DNA-DNA hybridization values between strain M65 and the type strains of species were 73.9-85.8 % and 19.9-22.4 %, respectively. The sole respiratory quinone detected in the three isolates was ubiquinone 10. The principal fatty acids were summed feature 8 (C 7 and/or C 6) and C. The major polar lipids were glucuronopyranosyldiglyceride, monoglycosyldiglyceride and one unidentified glycolipid. The DNA G+C content was 61.3-61.4 mol%. Phylogenetic distinctiveness, chemotaxonomic differences, together with phenotypic properties, revealed that the isolates could be differentiated from the species with validly published names. Therefore, it is proposed that strains M65, M69 and JN25 represent a novel species of the genus , for which the name sp. nov. (type strain, M65=CGMCC 1.15927=KCTC 52576) is proposed.
菌株M65、M69和JN25是从西太平洋海水中分离得到的。这三株菌株的细胞革兰氏染色阴性，好氧，呈杆状。细胞通过鞭毛运动。基于16S rRNA基因序列分析结果，菌株M65、M69和JN25与MCS27的16S rRNA基因序列相似性最高（98.8%），其次是DSM 19595（98.4%）、MCS23（98.4%）、LA220（98.3%）、P38（98.1%）和SD10（97.8%）。分离株之间的16S rRNA基因序列相似性为100%。系统发育分析表明，这些分离株属于包含该物种的一个簇，并代表一个独立的谱系。菌株M65与该物种模式菌株之间的平均核苷酸同一性和DNA-DNA杂交值分别为73.9 - 85.8%和19.9 - 22.4%。在这三株分离株中检测到的唯一呼吸醌是泛醌10。主要脂肪酸为总和特征8（C17:1ω6c和/或C17:1ω7c）和C16:0。主要极性脂质为葡糖醛酸吡喃糖基二甘油酯、单糖基二甘油酯和一种未鉴定的糖脂。DNA G + C含量为61.3 - 61.4 mol%。系统发育独特性、化学分类学差异以及表型特征表明，这些分离株可与已有效发表名称的该物种区分开来。因此，建议菌株M65、M69和JN25代表该属的一个新物种，为此提出新物种名称为[具体属名] sp. nov.（模式菌株，M65 = CGMCC 1.15927 = KCTC 52576）。
