Wu Yue-Hong, Cheng Hong, Huo Ying-Yi, Jin Xiong-Bin, Wang Chun-Sheng, Xu Xue-Wei
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3020-3025. doi: 10.1099/ijsem.0.002066. Epub 2017 Aug 18.
Strain LA220T, isolated from seawater of the Eastern Pacific Ocean, was subjected to a polyphasic taxonomic study. Cells of the strain were Gram-stain-negative, aerobic, motile and short rod-shaped. On the basis of 16S rRNA gene sequence analysis, strain LA220T showed high similarity to Henriciella litoralis SD10T (98.5 %), Henriciella marina DSM 19595T (98.3 %) and Henriciellaaquimarina P38T (97.5 %), and exhibited less than 97.0 % 16S rRNA gene sequence similarity with respect to the type strains of other Hyphomonadaceae species. Phylogenetic analyses revealed that strain LA220T fell within the cluster of the genus Henriciella. The average nucleotide identity and in silico DNA-DNA hybridization values between strain LA220T and the type strains of Henriciella species were 74.8-76.8 and 18.4-20.8 %, respectively. The sole respiratory quinone was ubiquinone-10 (Q-10). The principal fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The major polar lipids were three unidentified glycolipids. The DNA G+C content was 59.9 mol%. Phylogenetic distinctiveness, chemotaxonomic differences and phenotypic properties revealed that strain LA220T could be differentiated from recognized Henriciella species. Therefore, strain LA220T is considered to represent a novel species of the genus Henriciella, for which the name Henriciella pelagia sp. nov. (type strain LA220T=CGMCC 1.15928T=KCTC 52577T) is proposed.
从东太平洋海水分离出的菌株LA220T进行了多相分类研究。该菌株细胞革兰氏染色阴性，好氧，具运动性，呈短杆状。基于16S rRNA基因序列分析，菌株LA220T与滨海亨氏菌SD10T（98.5%）、海洋亨氏菌DSM 19595T（98.3%）和海生亨氏菌P38T（97.5%）显示出高度相似性，并且与其他生丝微菌科物种的模式菌株的16S rRNA基因序列相似性低于97.0%。系统发育分析表明，菌株LA220T属于亨氏菌属的聚类群。菌株LA220T与亨氏菌属各物种模式菌株之间的平均核苷酸同一性和电子计算机DNA-DNA杂交值分别为74.8 - 76.8%和18.4 - 20.8%。唯一的呼吸醌是泛醌-10（Q-10）。主要脂肪酸为总和特征8（C18:1ω7c和/或C18:1ω6c）和C16:0。主要极性脂类是三种未鉴定的糖脂。DNA G+C含量为59.9 mol%。系统发育独特性、化学分类学差异和表型特征表明，菌株LA220T可与已确认的亨氏菌属物种区分开来。因此，菌株LA220T被认为代表亨氏菌属的一个新物种，为此提出名称海栖亨氏菌（Henriciella pelagia sp. nov.）（模式菌株LA220T = CGMCC 1.15928T = KCTC 52577T）。
