Collaborative Innovation Center of Chemistry for Energy Material, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University , Xiamen 361005, P. R. China.
Anal Chem. 2017 Sep 19;89(18):9857-9863. doi: 10.1021/acs.analchem.7b01885. Epub 2017 Aug 29.
Semiconducting quantum dots (QDs) are finding a wide range of biomedical applications due to their intense fluorescence brightness and long-term photostability. Here, we report precise quantification of the fluorescence intensity of single QDs on a laboratory-built high-sensitivity flow cytometer (HSFCM). The nearly uniform illumination of the particles at the intense portions of the radiation field resulted in narrowly distributed signals with high signal-to-noise ratios. By analysis of thousands of QDs individually in as little time as 1 min, intrinsic polydispersity was quickly revealed in a statistically robust manner. Applications of this technique in QD quality assessment, study of metal ion influence, and evaluation of aggregation upon biomolecule coupling are presented. Moreover, an accurate measurement of the QD particle concentration was achieved via single-particle enumeration. HSFCM is believed to provide a powerful characterization tool for QD synthesis and application development.
半导体量子点（QDs）由于其强烈的荧光亮度和长期的光稳定性，正在找到广泛的生物医学应用。在这里，我们报告了在实验室构建的高灵敏度流动细胞仪（HSFCM）上对单个 QD 的荧光强度进行精确量化。在辐射场的强部分，粒子几乎均匀地照明导致了具有高信噪比的窄分布信号。通过在短短 1 分钟内对数千个 QD 进行单独分析，以统计上可靠的方式快速揭示了内在的多分散性。该技术在 QD 质量评估、金属离子影响研究以及生物分子偶联时聚集的评估中的应用也得到了介绍。此外，通过单粒子计数实现了 QD 颗粒浓度的精确测量。HSFCM 被认为为 QD 的合成和应用开发提供了一种强大的表征工具。
