College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Biosens Bioelectron. 2018 Jan 15;99:458-463. doi: 10.1016/j.bios.2017.08.010. Epub 2017 Aug 10.
Herein, bimetallic dendritic core-shell AuPd@Au nanocrystals (AuPd@Au NCs) were prepared by a simple one-pot aqueous method using xanthine as a green growth-directing agent. By virtue of the enhanced peak currents in the HO reduction catalyzed by AuPd@Au NCs, a label-free immunosensor was constructed for the detection of prostate specific antigen (PSA). The immunosensor exhibited significantly improved analytical performance for the assay of PSA with wide linear range of 0.1-50ngmL and low detection limit of 0.078ngmL (S/N = 3), coupled with the improved stability, reproducibility and selectivity. It provides a promising platform for clinical research and diagnosis.
在此，通过使用黄嘌呤作为绿色导向生长剂的简单一锅水相法制备了双金属枝状核壳型 AuPd@Au 纳米晶体（AuPd@AuNCs）。由于 AuPd@AuNCs 催化 HO 还原的峰电流增强，构建了用于检测前列腺特异性抗原（PSA）的无标记免疫传感器。该免疫传感器在 PSA 的测定中表现出显著改善的分析性能，具有较宽的线性范围为 0.1-50ngmL 和较低的检测限为 0.078ngmL（S/N = 3），同时还具有改善的稳定性、重现性和选择性。它为临床研究和诊断提供了一个很有前途的平台。
