Key laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Key laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Biosens Bioelectron. 2018 Jul 15;111:47-51. doi: 10.1016/j.bios.2018.03.052. Epub 2018 Mar 23.
Herein, well-defined hollow dendritic AuPtAg alloyed nanocrystals (ANCs) were synthesized by a simple L-proline-mediated one-pot aqueous method. More importantly, the synthesized hollow dendritic architectures provide a suitable platform for immobilization of anti-prostate specific antigen (PSA). The resultant label-free immunosensor exhibited the improved performance for highly sensitive detection of PSA based on the enhanced catalytic currents of K[Fe(CN)] as a signal probe. Impressively, the immunosensor showed the wide linear range of 0.05-50 ng mL and low detection limit of 0.017 ng mL under optimal conditions for the assay of PSA, couple with the improved stability, reproducibility and selectivity. It provides a promising platform for clinical research and diagnosis.
在此，通过一种简单的 L-脯氨酸介导的一锅水相法合成了具有明确空心树枝状结构的 AuPtAg 合金纳米晶体（ANCs）。更重要的是，所合成的空心树枝状结构为固定抗前列腺特异性抗原（PSA）提供了合适的平台。基于增强的 K[Fe(CN)]作为信号探针的催化电流，所得无标记免疫传感器在高灵敏度检测 PSA 方面表现出了改进的性能。引人注目的是，在最佳测定条件下，该免疫传感器对 PSA 的检测具有较宽的线性范围（0.05-50ng/mL）和较低的检测限（0.017ng/mL），并且具有改善的稳定性、重现性和选择性。它为临床研究和诊断提供了一个有前途的平台。
