Lockwood C M, Bakes D, Jones S, Whitaker K B, Moss D W, Savage C O
Lancet. 1987 Mar 28;1(8535):716-20. doi: 10.1016/s0140-6736(87)90357-6.
A solid phase radioimmunoassay has been developed to detect circulating autoantibodies to neutrophil cytoplasmic antigens in systemic vasculitis. After fractionation of these antigens by size, with gel filtration high performance liquid chromatography, sera from patients with clinically different forms of systemic vasculitis, Wegener's granulomatosis (WG) and microscopic polyarteritis (MP), showed contrasting specificities of binding. WG sera bound to 100, 6.2, and 1.8 kD components, whereas MP sera bound only to the 100 kD component, allowing immunological distinction between the syndromes. The 100 kD component recognised by both WG and MP sera also showed alkaline phosphatase activity. Further evidence for this association was obtained by direct binding experiments between systemic vasculitis sera and calf-intestinal or human neutrophil alkaline phosphatase and by the cross-reactivity of W8, a monoclonal antibody raised to a neutrophil cytoplasmic autoantigen, with various preparations of the enzyme.
已开发出一种固相放射免疫分析法，用于检测系统性血管炎中针对中性粒细胞胞浆抗原的循环自身抗体。在用凝胶过滤高效液相色谱法按大小对这些抗原进行分级分离后，来自具有临床不同形式的系统性血管炎、韦格纳肉芽肿（WG）和显微镜下多动脉炎（MP）患者的血清显示出不同的结合特异性。WG血清与100、6.2和1.8 kD成分结合，而MP血清仅与100 kD成分结合，从而实现了这两种综合征之间的免疫学区分。WG和MP血清均识别的100 kD成分也显示出碱性磷酸酶活性。通过系统性血管炎血清与小牛肠或人中性粒细胞碱性磷酸酶之间的直接结合实验，以及针对中性粒细胞胞浆自身抗原产生的单克隆抗体W8与各种酶制剂的交叉反应，获得了这种关联的进一步证据。
