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微小 RNA 218a-5p、219a-5p 和 221-3p 调节前庭代偿。

MicroRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation.

机构信息

Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, 06973, Republic of Korea.

Department of Otolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.

出版信息

Sci Rep. 2017 Aug 18;7(1):8701. doi: 10.1038/s41598-017-09422-8.

DOI:10.1038/s41598-017-09422-8
PMID:28821887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5562769/
Abstract

Unilateral vestibular deafferentation (UVD) interrupts afferent signals from one side, resulting in an imbalance of the resting activity between bilateral vestibular nuclei. Vestibular compensation is the process of balancing the resting activity to reestablish homeostasis. Here, we investigated microRNAs (miRNAs) that regulate vestibular compensation using the Sprague-Dawley rat. After determining the progression of vestibular compensation following UVD, microarray analysis was performed and nine miRNAs were selected as candidates. Following validation by quantitative reverse transcription-PCR, three miRNAs remained. We assessed the effect of these miRNAs on vestibular compensation using miRNA oligomers. We compared the results of the rotarod test and 5-bromo-2'-deoxyuridine immunohistochemistry following UVD between the control group and the groups in which the candidate miRNA oligomers were administered. Administration of miR-218a-5p, 219a-5p, and 221-3p oligomers significantly affected vestibular compensation. Target pathway analysis of these miRNAs supported our results. Our findings suggest that the miRNAs 218a-5p, 219a-5p, and 221-3p regulate vestibular compensation.

摘要

单侧前庭去传入（UVD）中断了来自一侧的传入信号，导致双侧前庭核之间的静息活动失衡。前庭代偿是平衡静息活动以重新建立体内平衡的过程。在这里，我们使用 Sprague-Dawley 大鼠研究了调节前庭代偿的 microRNAs（miRNAs）。在确定 UVD 后前庭代偿的进展后，进行了微阵列分析，并选择了 9 个 miRNAs 作为候选物。通过定量逆转录-PCR 验证后，有 3 个 miRNA 保留下来。我们使用 miRNA 寡核苷酸评估了这些 miRNA 对前庭代偿的影响。我们比较了 UVD 后对照组和给予候选 miRNA 寡核苷酸组的旋转棒测试和 5-溴-2'-脱氧尿苷免疫组织化学的结果。miR-218a-5p、219a-5p 和 221-3p 寡核苷酸的给药显著影响了前庭代偿。这些 miRNA 的靶通路分析支持了我们的结果。我们的研究结果表明，miRNAs 218a-5p、219a-5p 和 221-3p 调节前庭代偿。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/bcb5d83da4a1/41598_2017_9422_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/7c2557d59b9f/41598_2017_9422_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/af0f0bdd56af/41598_2017_9422_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/d8d6ee7f1c50/41598_2017_9422_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/4e9668ba6d93/41598_2017_9422_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/bcb5d83da4a1/41598_2017_9422_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/7c2557d59b9f/41598_2017_9422_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/af0f0bdd56af/41598_2017_9422_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/d8d6ee7f1c50/41598_2017_9422_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/4e9668ba6d93/41598_2017_9422_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8d/5562769/bcb5d83da4a1/41598_2017_9422_Fig5_HTML.jpg

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