Rodrigues Janderson Teixeira, Dos Santos Antunes Henrique, Armada Luciana, Pires Fábio Ramôa
Post-graduation Program in Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
Department of Endodontics, School of Dentistry, Grande Rio University, Rio de Janeiro, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2017 Dec;124(6):561-567. doi: 10.1016/j.oooo.2017.06.121. Epub 2017 Jul 5.
The biologic effects of surgical decompression on the epithelium and connective tissues of periapical cysts are not fully understood. The aim of this study was to evaluate the expression of tissue repair and inflammatory biomarkers in periapical cysts before and after surgical decompression.
Nine specimens of periapical cysts treated with decompression before undergoing complete enucleation were immunohistochemically analyzed to investigate the expression of interleukin-1β, tumor necrosis factor-α, transforming growth factor-β1, matrix metalloproteinase-9, Ki-67, and epidermal growth factor receptor. Expression of the biomarkers was classified as positive, focal, or negative. Ki-67 immunoexpression was calculated as a cell proliferation index. The expression of the biomarkers was compared in the specimens from decompression and from the final surgical procedure.
Computed tomography demonstrated that volume was reduced in all cysts after decompression. There were no differences in the immunoexpression of the proinflammatory and tissue repair biomarkers when comparing the specimens obtained before and after the decompression.
Surgical decompression was efficient in reducing the volume of periapical cysts before complete enucleation. When comparing the specimens obtained from surgical decompression and from complete surgical removal, the immunohistochemical analysis did not show a decrease in proinflammatory biomarkers; neither did it show an increase in tissue repair biomarkers.
手术减压对根尖囊肿上皮和结缔组织的生物学效应尚未完全明确。本研究旨在评估手术减压前后根尖囊肿中组织修复和炎症生物标志物的表达情况。
对9例在完全摘除术前接受减压治疗的根尖囊肿标本进行免疫组织化学分析，以研究白细胞介素-1β、肿瘤坏死因子-α、转化生长因子-β1、基质金属蛋白酶-9、Ki-67和表皮生长因子受体的表达。生物标志物的表达分为阳性、局灶性或阴性。计算Ki-67免疫表达作为细胞增殖指数。比较减压标本和最终手术标本中生物标志物的表达。
计算机断层扫描显示减压后所有囊肿体积均减小。比较减压前后获得的标本，促炎和组织修复生物标志物的免疫表达无差异。
手术减压在完全摘除术前有效减小了根尖囊肿的体积。比较手术减压和完全手术切除获得的标本，免疫组织化学分析未显示促炎生物标志物减少，也未显示组织修复生物标志物增加。
