Ecotoxicological effects of binary mixtures of siduron and Cd on mRNA expression in the earthworm Eisenia fetida.

This study aimed to investigate the eco-toxicological responses of earthworm (Eisenia fetida) exposed to combined siduron (herbicide) and cadmium (Cd). Eisenia fetida gene expressions including metallothionein (MT) and heat shock protein70 (Hsp70) were analyzed using real-time Polymerase Chain Reaction after individual and combined siduron (0.90, 1.80, 3.60 and 7.20μgcm) and Cd (0.225, 0.45, 0.90 and 1.80μgcm) sublethal exposures. Where, the nature of the toxicological interactions between siduron and Cd in inducing or suppressing MT and Hsp70 expression was determined by applying the Combination index (CI)-isobologram model. The results revealed significant variations in MT and weak changes in Hsp70 expression when the earthworms were exposed to individual Cd. The individual siduron exposure exhibited a significant down-regulation (p<0.01) in MT during all treatments and in Hsp70 expression only at 7.20μgcm concentration; while the mixtures of siduron and Cd exposures resulted a significant down regulation (p<0.05) in both MT and Hsp70 expressions. Moreover, the combined siduron and Cd exposure revealed nearly additive effect (CI=1) at the lower effect levels and significant synergistic effect (CI<1) at the higher effect levels for both MT and Hsp70 expression. The synergistic effects of combined siduron and Cd suggest that there might be a potential risk connected to the co-occurrence of these chemicals in the environment.