State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, PR China; University of Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing 100049, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, PR China.
Biosens Bioelectron. 2018 Jan 15;99:519-524. doi: 10.1016/j.bios.2017.08.028. Epub 2017 Aug 12.
N-hydroxyphthalimide (NHPI), a well known reagent in organic synthesis and biochemical applications, has been developed as a stable and efficient chemiluminescence coreactant for the first time. It reacts with luminol much faster than N-hydroxysuccinimide, eliminating the need of a prereaction coil used in N-hydroxysuccinimide system. Without using prereaction coil, the chemiluminescence peak intensities of luminol-NHPI system are about 102 and 26 times greater than that of luminol-N-hydroxysuccinimide system and classical luminol-hydrogen peroxide system, respectively. The luminol-NHPI system achieves the highly sensitive detection of luminol (LOD = 70pM) and NHPI (LOD = 910nM). Based on their excellent quenching efficiencies, superoxide dismutase and uric acid are sensitively detected with LODs of 3ng/mL and 10pM, respectively. Co is also detected a LOD of 30pM by its remarkable enhancing effect. Noteworthily, our method is at least 4 orders of magnitude more sensitive than previously reported uric acid detection methods, and can detect uric acid in human urine and Co in tap and lake water real samples with excellent recoveries in the range of 96.35-102.70%. This luminol-NHPI system can be an important candidate for biochemical, clinical and environmental analysis.
N-羟基邻苯二甲酰亚胺（NHPI）作为一种在有机合成和生物化学应用中广为人知的试剂，首次被开发为一种稳定且高效的化学发光共反应物。它与鲁米诺的反应速度比 N-羟基琥珀酰亚胺快得多，因此不需要在 N-羟基琥珀酰亚胺体系中使用预反应盘管。不使用预反应盘管时，鲁米诺-NHPI 体系的化学发光峰强度比鲁米诺-N-羟基琥珀酰亚胺体系和经典的鲁米诺-过氧化氢体系分别大约 102 和 26 倍。鲁米诺-NHPI 体系实现了对鲁米诺（LOD=70pM）和 NHPI（LOD=910nM）的高灵敏检测。基于其出色的猝灭效率，超氧化物歧化酶和尿酸的检测限分别达到 3ng/mL 和 10pM。Co 也因其显著的增强效应而被检测到 LOD 为 30pM。值得注意的是，与之前报道的尿酸检测方法相比，我们的方法至少灵敏了 4 个数量级，并且可以检测到人体尿液中的尿酸和自来水中及湖水中的 Co，实际样品的回收率在 96.35-102.70%范围内。该鲁米诺-NHPI 体系有望成为生物化学、临床和环境分析的重要候选试剂。
