School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, Shenyang, Liaoning Province, 110016, People's Republic of China.
Mikrochim Acta. 2022 Jul 19;189(8):283. doi: 10.1007/s00604-022-05381-8.
An efficient analysis platform composed of nanozyme-based hydrogel kit and smartphone was constructed for on-site detection of uric acid (UA) in a rapid and realiable manner. CuCoS nanoparticles (CuCoS NPs) as a peroxidase mimic were successfully prepared and the peroxidase-like activity and catalytic mechanism were studied in detail. The hydrogen peroxide (HO) stimulus-responsive nanozyme-based hydrogel kit was manufactured by integrating agarose, CuCoS NPs, and 3,3',5,5'-tetramethylbenzidine (TMB) into the cap of centrifuge tube. HO generated via UA oxidation acts as stimulus signal, which triggers the oxidation of TMB to form blue product (oxTMB) under the catalysis of CuCoS NPs, resulting in the color response of the constructed kit. The color image of the kit was captured by a smartphone built-in camera and converted into color intensity using ImageJ software, thus achieving the quantitative determination of UA. The portable kit possesses high selectivity and was used to monitor UA in human serum with satisfactory results (recovery was in the range 95.8-107.3% and RSD was not greater than 4.6%). The established sensing platform is convenient and reliable, which provides a new strategy for point-of-care testing of UA and has a broad prospect in the fields of chemical sensing and biomedical.
构建了一种由基于纳米酶的水凝胶试剂盒和智能手机组成的高效分析平台，用于现场快速可靠地检测尿酸 (UA)。成功制备了作为过氧化物酶模拟物的 CuCoS 纳米粒子 (CuCoS NPs)，并详细研究了其过氧化物酶样活性和催化机制。通过将琼脂糖、CuCoS NPs 和 3,3',5,5'-四甲基联苯胺 (TMB) 整合到离心管的盖子中，制造了对过氧化氢 (HO) 刺激响应的基于纳米酶的水凝胶试剂盒。UA 氧化产生的 HO 作为刺激信号，在 CuCoS NPs 的催化下，将 TMB 氧化生成蓝色产物 (oxTMB)，从而导致构建的试剂盒发生颜色响应。试剂盒的颜色图像由智能手机内置摄像头捕获，并使用 ImageJ 软件转换为颜色强度，从而实现 UA 的定量测定。该便携式试剂盒具有高选择性，用于监测人血清中的 UA，结果令人满意（回收率在 95.8-107.3%之间，RSD 不大于 4.6%）。所建立的传感平台方便可靠，为即时检测 UA 提供了新策略，在化学传感和生物医学领域具有广阔的前景。
