Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan.
Department of Forestry and Natural Resources, National Chiayi University, Chiayi 600, Taiwan.
Int J Med Sci. 2017 Jul 19;14(9):862-870. doi: 10.7150/ijms.19553. eCollection 2017.
Some members of genus are traditionally used as medicinal plants for arthritis, acute and chronic bronchitis, asthma, pain, inflammation, rheumatism, hypertension and metabolic diseases. To the best of our knowledge, there is no report on the protective effects of leaf extract on non-alcoholic fatty liver disease (NAFLD) and . In this study, the effects of leaf extract on inhibiting the free fatty acid (FFA)-induced accumulation of fat in HepG2 cells and on improving fatty liver syndrome in mice with high fat diet (HFD)-induced NAFLD were investigated. For the assay, HepG2 cells were treated with FFAs (oleate/palmitate = 2:1) with or without treatment with leaf ethyl acetate (EtOAc) fraction to observe lipid accumulation using Nile red and oil red O stains. For the assay, C57BL/6 mice were randomly assigned to three groups ( = 5), including the normal diet group, the HFD group and the HFD+EtOAc group. After 11 weeks, body weight, serum biochemical indices and the mRNA expressions of the liver tissue, as well as the outward appearance, weight and histopathological analysis of liver and adipose tissues were evaluated. Among the fractions derived from leaf, the EtOAc fraction exhibited a strong fat-accumulation inhibitory activity. Following reverse-phase high-performance liquid chromatography (HPLC), four specific phytochemicals, including (2, 3)-astilbin (AS), hyposide (HY), guaijaverin (GU) and quercitrin (QU), were isolated and identified from the EtOAc fraction of leaf extract. Among them, AS and HY showed excellent fat-accumulation inhibitory activity. Thus, the EtOAc fraction of leaf and its derived phytochemicals have great potential in preventing FFA-induced fat accumulation. In addition, the EtOAc fraction of leaf significantly improved fatty liver syndrome and reduced total cholesterol (TC) and triglyceride (TG) in HFD-induced NAFLD mice at a dosage of 200 mg/kg BW. These results demonstrated that the methanolic extracts from leaf have excellent inhibitory activities against fat accumulation and anti-NAFLD activities and thus have great potential as a natural health product.
一些属的成员传统上被用作治疗关节炎、急性和慢性支气管炎、哮喘、疼痛、炎症、风湿、高血压和代谢疾病的药用植物。据我们所知，目前还没有关于 叶提取物对非酒精性脂肪肝疾病（NAFLD）和 的保护作用的报道。在这项研究中，我们研究了 叶提取物对抑制游离脂肪酸（FFA）诱导的 HepG2 细胞脂肪堆积的作用，以及对高脂肪饮食（HFD）诱导的非酒精性脂肪肝（NAFLD）小鼠脂肪性肝病综合征的改善作用。在 实验中，用油酸/棕榈酸（2:1）处理 HepG2 细胞，并用或不用 叶乙酸乙酯（EtOAc）馏分处理，用尼罗红和油红 O 染色观察脂滴积累。在 实验中，将 C57BL/6 小鼠随机分为三组（每组 5 只），包括正常饮食组、HFD 组和 HFD+EtOAc 组。11 周后，评估体重、血清生化指标、肝组织 mRNA 表达以及肝和脂肪组织的外观、重量和组织病理学分析。在 叶衍生的馏分中，EtOAc 馏分表现出很强的脂肪积累抑制活性。通过反相高效液相色谱（HPLC），从 叶乙酸乙酯馏分中分离并鉴定出四种特定的植物化学物质，包括（2,3）-astilbin（AS）、hyposide（HY）、guaijaverin（GU）和 quercitrin（QU）。其中，AS 和 HY 表现出优异的脂肪积累抑制活性。因此， 叶的 EtOAc 馏分及其衍生的植物化学物质在预防 FFA 诱导的脂肪积累方面具有很大的潜力。此外，200mg/kgBW 的 EtOAc 馏分可显著改善 HFD 诱导的 NAFLD 小鼠的脂肪性肝病综合征，并降低总胆固醇（TC）和甘油三酯（TG）。这些结果表明， 叶的甲醇提取物具有优异的脂肪积累抑制活性和抗非酒精性脂肪肝（NAFLD）活性，因此具有作为天然保健品的巨大潜力。
