Kianmehr Majid, Haghmorad Dariush, Nosratabadi Reza, Rezaei Abdolrahim, Alavinezhad Azam, Boskabady Mohammad Hossein
Neurogeneeic Inflammation Research Centre, Mashhad University of Medical SciencesMashhad, Iran.
Department of Physiology, School of Medicine, Mashhad University of Medical SciencesMashhad, Iran.
Front Pharmacol. 2017 Aug 7;8:458. doi: 10.3389/fphar.2017.00458. eCollection 2017.
Asthma is a chronic inflammatory disease with no definite treatment and more research is needed to overcome this condition. The aim of this study was to investigate the effect of the extract of () as a medicinal plant on cytokine genes expression in an experimental mouse model of asthma. Adult mice were randomly divided into the following groups: control (C), untreated asthma (A), asthmatic groups treated with dexamethasone (D) and extract (200, 400, and 800 μg/mL; Z1, Z2, and Z3, respectively), (for groups C, A, and D = 5 and for groups Z1, Z2, and Z3 = 6). For induction of the mouse model of asthma, animals were sensitized with intraperitoneal injection and inhalation of ovalbumin (OVA). The number of T helper (Th) subtype cells (using flow cytometry) and the levels of IFN-γ, FOXP3, IL-4, TGF-β, IL-17 gene expression (by real time PCR) were assessed in mice splenocytes. The observed changes in spleen cells of group A compared to group C were increased number of Th and Th cells, enhancement of gene expression of IL-4, IL-17, and TGF-β ( < 0.001 for all cases), reduction of Th cells and Th/Th ratio ( < 0.001 for both cases) and decrease in gene expression of IFN-γ, FOXP and IFN-γ/IL-4 ratio ( < 0.01 for IFN-γ and < 0.001 for other cases). The observed changes in spleen cells of treated compared to untreated A group were enhancement of Treg cells and Th/Th ratio ( < 0.001 for both cases), increase in IFN-γ ( < 0.05) and FOXP ( < 0.001) gene expression and IFN-γ/IL-4 ratio ( < 0.01) as well as reduction of Th and Th cells ( < 0.01 to < 0.001), decrease gene expression of IL-4, IL-17, and TGF-β ( < 0.05 to < 0.001). The findings showed that the extract of decreased pro-inflammatory cytokines in asthma (IL-4 and IL-17 and TGF-β) but increased anti-inflammatory cytokines (IFN-γ) gene expression and the number of Treg (FOXP3) in splenocytes of asthmatic mice which may indicate the specific therapeutic effect of the plant extract in allergy, autoimmunity, and infectious diseases via potentiating Th and suppressing Th2 and Th cells.
哮喘是一种慢性炎症性疾病，尚无确切的治疗方法，需要更多的研究来攻克这一病症。本研究的目的是在哮喘实验小鼠模型中，研究作为药用植物的（此处原文缺失植物名称）提取物对细胞因子基因表达的影响。成年小鼠被随机分为以下几组：对照组（C）、未治疗的哮喘组（A）、用地塞米松治疗的哮喘组（D）以及提取物治疗组（200、400和800μg/mL；分别为Z1、Z2和Z3组），（C组、A组和D组每组n = 5，Z1组、Z2组和Z3组每组n = 6）。为诱导哮喘小鼠模型，通过腹腔注射和吸入卵清蛋白（OVA）使动物致敏。在小鼠脾细胞中评估辅助性T（Th）亚型细胞数量（采用流式细胞术）以及IFN-γ、FOXP3、IL-4、TGF-β、IL-17基因表达水平（通过实时PCR）。与C组相比，A组脾细胞观察到的变化为Th和Th细胞数量增加，IL-4、IL-17和TGF-β基因表达增强（所有情况均P < 0.001），Th细胞数量和Th/Th比值降低（两种情况均P < 0.001），IFN-γ、FOXP和IFN-γ/IL-4比值基因表达降低（IFN-γ为P < 0.01，其他情况为P < 0.001）。与未治疗的A组相比，治疗组脾细胞观察到的变化为调节性T（Treg）细胞和Th/Th比值增强（两种情况均P < 0.001），IFN-γ（P < 0.05）和FOXP（P < 0.001）基因表达以及IFN-γ/IL-4比值增加（P < 0.01），同时Th和Th细胞数量减少（P < 0.01至P < 0.001），IL-4、IL-17和TGF-β基因表达降低（P < 0.05至P < 0.001）。研究结果表明，（此处原文缺失植物名称）提取物可降低哮喘中的促炎细胞因子（IL-4、IL-17和TGF-β），但增加抗炎细胞因子（IFN-γ）基因表达以及哮喘小鼠脾细胞中Treg（FOXP3）细胞数量，这可能表明该植物提取物通过增强Th细胞并抑制Th2和Th细胞，在过敏、自身免疫和传染病中具有特定的治疗作用。
